Carbon monoxide as a putative messenger molecule in the feline lower oesophageal sphincter of the cat

Research output: Contribution to journalArticle

Abstract

The distribution of the carbon monoxide (CO) producing enzymes haem oxygenase (HO) type 1 and 2 were studied in the feline lower oesophageal sphincter (LOS), as were HO activity and functional effects of CO. HO-2 immunoreactivity was observed in nerve cell bodies in the submucosal and myenteric plexus, nerve fibres, non-neuronal cells surrounding smooth muscle bundles, and in arterial endothelium, HO-1 immunoreactivity was confined to non-neuronal cells in the smooth muscle layer. CO production, indicating HO activity, was demonstrated in tissue homogenates. CO relaxed the LOS, and activated the cyclic GMP system. These results show that HO is present in the LOS, and suggest that CO can be generated by neuronal and non-neuronal structures and may have a role as a peripheral messenger.

Details

Authors
Organisations
External organisations
  • Skåne University Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Pharmacology and Toxicology

Keywords

  • Animals, Carbon Monoxide/metabolism, Cats, Cyclic AMP/metabolism, Cyclic GMP/metabolism, Enzyme Inhibitors/pharmacology, Esophagogastric Junction/drug effects, Heme Oxygenase (Decyclizing)/antagonists & inhibitors, Immunohistochemistry, Isoenzymes/antagonists & inhibitors, Male, Microscopy, Confocal, Muscle Contraction/drug effects, Muscle, Smooth/drug effects, Protoporphyrins/pharmacology
Original languageEnglish
Pages (from-to)1389-1393
JournalNeuroReport
Volume6
Issue number10
Publication statusPublished - 1995 Jul
Publication categoryResearch
Peer-reviewedYes