Cartilage oligomeric matrix protein contributes to the development and metastasis of breast cancer

Research output: Contribution to journalArticle

Abstract

Cartilage oligomeric matrix protein (COMP) is a soluble pentameric protein expressed in cartilage and involved in collagen organization. Tissue microarrays derived from two cohorts of patients with breast cancer (n=122 and n=498) were immunostained, revealing varying expression of COMP, both in the tumor cells and surrounding stroma. High levels of COMP in tumor cells correlated, independently of other variables, with poor survival and decreased recurrence-free survival. Breast cancer cells, MDA-MB-231, stably expressing COMP were injected into the mammary fat pad of SCID (CB-17/Icr-Prkdc(scid)/Rj) mice. Tumors expressing COMP were significantly larger and were more prone to metastasize as compared with control, mock-transfected, tumors. In vitro experiments confirmed that COMP-expressing cells had a more invasive phenotype, which could in part be attributed to an upregulation of matrix metalloprotease-9. Furthermore, microarray analyses of gene expression in tumors formed in vivo showed that COMP expression induced higher expression of genes protecting against endoplasmic reticulum stress. This observation was confirmed in vitro as COMP-expressing cells showed better survival as well as a higher rate of protein synthesis when treated with brefeldin A, compared with control cells. Further, COMP-expressing cells appeared to undergo a metabolic switch, that is, a Warburg effect. Thus, in vitro measurement of cell respiration indicated decreased mitochondrial metabolism. In conclusion, COMP is a novel biomarker in breast cancer, which contributes to the severity of the disease by metabolic switching and increasing invasiveness and tumor cell viability, leading to reduced survival in animal models and human patients.

Details

Authors
Organisations
External organisations
  • Medical University of Gdansk
  • Juntendo University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cancer and Oncology

Keywords

  • Animals, Apoptosis, Biomarkers, Tumor, Breast Neoplasms, Cartilage Oligomeric Matrix Protein, Cell Adhesion, Cell Line, Cell Membrane, Cell Movement, Cell Transformation, Neoplastic, Disease Models, Animal, Endoplasmic Reticulum, Endoplasmic Reticulum Stress, Female, Gene Expression, Gene Expression Profiling, Heterografts, Humans, Immunohistochemistry, Matrix Metalloproteinase 9, Mice, SCID, Neoplasm Metastasis, Oxidative Phosphorylation, Prognosis, Proportional Hazards Models, Recurrence, Journal Article
Original languageEnglish
Pages (from-to)5585-5596
Number of pages12
JournalOncogene
Volume35
Issue number43
Publication statusPublished - 2016
Publication categoryResearch
Peer-reviewedYes

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