CD103 + CD11b + mucosal classical dendritic cells initiate long-term switched antibody responses to flagellin

Research output: Contribution to journalArticle

Abstract

Antibody responses induced at mucosal and nonmucosal sites demonstrate a significant level of autonomy. Here, we demonstrate a key role for mucosal interferon regulatory factor-4 (IRF4)-dependent CD103 + CD11b + (DP), classical dendritic cells (cDCs) in the induction of T-dependent immunoglobulin G (IgG) and immunoglobulin A (IgA) responses in the mesenteric lymph node (MLN) following systemic immunization with soluble flagellin (sFliC). In contrast, IRF8-dependent CD103 + CD11b ' (SP) are not required for these responses. The lack of this response correlated with a complete absence of sFliC-specific plasma cells in the MLN, small intestinal lamina propria, and surprisingly also the bone marrow (BM). Many sFliC-specific plasma cells accumulating in the BM of immunized wild-type mice expressed α 4 β 7 +, suggesting a mucosal origin. Collectively, these results suggest that mucosal DP cDC contribute to the generation of the sFliC-specific plasma cell pool in the BM and thus serve as a bridge linking the mucosal and systemic immune system.

Details

Authors
  • A. Flores-Langarica
  • K. Müller Luda
  • E. K. Persson
  • C. N. Cook
  • S. Bobat
  • J. L. Marshall
  • M. W. Dahlgren
  • K. Hägerbrand
  • K. M. Toellner
  • M. D. Goodall
  • D. R. Withers
  • I. R. Henderson
  • B. Johansson Lindbom
  • A. F. Cunningham
  • W. W. Agace
Organisations
External organisations
  • University of Birmingham
  • Technical University of Denmark
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area
Original languageEnglish
Pages (from-to)681-692
Number of pages12
JournalMucosal Immunology
Volume11
Issue number3
Publication statusPublished - 2018 May 1
Publication categoryResearch
Peer-reviewedYes