CDK2 is required by MYC to induce apoptosis

Research output: Contribution to journalArticle

Abstract

Depending upon the cellular and physiologic context, the overexpression of the MYC proto-oncogene results in rapid cell growth, proliferation, induction of apoptosis and/or proliferative arrest. What determines the precise consequences upon MYC activation is not clear. We have found that cyclin-dependent kinase 2 (CDK2) is required by MYC to induce apoptosis. MYC-induced apoptosis was suppressed in mouse embryonic fibroblasts (MEF) knocked out for Cdk2 or normal human fibroblasts (NHF) upon expression of the CDK2 inhibitor p27 or treated with RNAi directed at CDK2. Knockout of Cdk2 did not prevent MYC from inducing p53 and Bim. The inhibition of CDK2 did not prevent apoptosis induced by the DNA damaging agent etoposide. Our results surprisingly suggest that CDK2 defines whether MYC induction causes apoptosis.

Details

Authors
External organisations
  • Stanford University
  • National Cancer Institute at Frederick
Research areas and keywords

Keywords

  • Apoptosis, CDK2, Cyclin E, MYC, p27
Original languageEnglish
Pages (from-to)1342-1347
JournalCell Cycle
Volume5
Issue number12
Publication statusPublished - 2006 Jun 15
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes