Cell and tissue manipulation with ultrashort infrared laser pulses in light-sheet microscopy

Research output: Contribution to journalArticle


Three-dimensional live imaging has become an indispensable technique in the fields of cell, developmental and neural biology. Precise spatio-temporal manipulation of biological entities is often required for a deeper functional understanding of the underlying biological process. Here we present a home-built integrated framework and optical design that combines three-dimensional light-sheet imaging over time with precise spatio-temporal optical manipulations induced by short infrared laser pulses. We demonstrate their potential for sub-cellular ablation of neurons and nuclei, tissue cauterization and optogenetics by using the Drosophila melanogaster and zebrafish model systems.


  • Gustavo de Medeiros
  • Dimitri Kromm
  • Balint Balazs
  • Nils Norlin
  • Stefan Günther
  • Emiliano Izquierdo
  • Paolo Ronchi
  • Shinya Komoto
  • Uros Krzic
  • Yannick Schwab
  • Francesca Peri
  • Stefano de Renzis
  • Maria Leptin
  • Matteo Rauzi
  • Lars Hufnagel
External organisations
  • European Molecular Biology Laboratory Heidelberg
  • Friedrich Miescher Institute for Biomedical Research
  • University of Zurich
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology
Original languageEnglish
Pages (from-to)1942
JournalScientific Reports
Issue number1
Publication statusPublished - 2020 Feb 6
Publication categoryResearch
Externally publishedYes