Cell-context signalling

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Cell-context signalling. / Pennell, Roger I.; Cronk, Quentin C. B.; Forsberg, L. Scott; Stöhr, Christine; Snogerup, Lars; Kjellbom, Per; McCabe, Paul F.

In: Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 350, No. 1331, 1995, p. 87-93.

Research output: Contribution to journalArticle

Harvard

Pennell, RI, Cronk, QCB, Forsberg, LS, Stöhr, C, Snogerup, L, Kjellbom, P & McCabe, PF 1995, 'Cell-context signalling', Philosophical Transactions of the Royal Society B: Biological Sciences, vol. 350, no. 1331, pp. 87-93. https://doi.org/10.1098/rstb.1995.0142

APA

Pennell, R. I., Cronk, Q. C. B., Forsberg, L. S., Stöhr, C., Snogerup, L., Kjellbom, P., & McCabe, P. F. (1995). Cell-context signalling. Philosophical Transactions of the Royal Society B: Biological Sciences, 350(1331), 87-93. https://doi.org/10.1098/rstb.1995.0142

CBE

Pennell RI, Cronk QCB, Forsberg LS, Stöhr C, Snogerup L, Kjellbom P, McCabe PF. 1995. Cell-context signalling. Philosophical Transactions of the Royal Society B: Biological Sciences. 350(1331):87-93. https://doi.org/10.1098/rstb.1995.0142

MLA

Vancouver

Author

Pennell, Roger I. ; Cronk, Quentin C. B. ; Forsberg, L. Scott ; Stöhr, Christine ; Snogerup, Lars ; Kjellbom, Per ; McCabe, Paul F. / Cell-context signalling. In: Philosophical Transactions of the Royal Society B: Biological Sciences. 1995 ; Vol. 350, No. 1331. pp. 87-93.

RIS

TY - JOUR

T1 - Cell-context signalling

AU - Pennell, Roger I.

AU - Cronk, Quentin C. B.

AU - Forsberg, L. Scott

AU - Stöhr, Christine

AU - Snogerup, Lars

AU - Kjellbom, Per

AU - McCabe, Paul F.

PY - 1995

Y1 - 1995

N2 - In plants, cells differentiate according to their position with relation to their cell neighbours. Monoclonal antibody (MAb) probes to polysaccharide epitopes, present at the surfaces of all plant cells, have defined a family of proteoglycan antigens which signify cellular position. These MAbs have been used to sort the single cells present in carrot somatic cell cultures on the basis of the presence or absence of specific polysaccharide epitopes. This sorting allows embryo initial cells to be cultured among different cell collectives (based on their polysaccharide epitope expression) and thus in altered contextual backgrounds. These experiments have shown that specific populations of embryo initial precursor cells induce and sustain the early development of the embryo initials, revealing that the populations of different cell collectives which are defined by different polysaccharide epitopes (cell-context) serves important regulatory function in early plant development. Somatic embryo initials deprived of the influence of the cell collective — defined by the presence of the polysaccharide epitope recognised by the MAb JIM8 — establish unorganised first divisions and develop as callus. However, in the presence of the JIM8-reactive cell collective, or medium conditioned by the collective, the initials develop into somatic embryos. This demonstrates that the cells defined by the JIM8 polysaccharide epitope are necessary to sustain the meristematic activity which drives the renewed development. Transfer of a cell-wall signal from the JIM8- reactive cells to cellular situations in carrot seedlings in which they would not normally occur (out-of-context signals) stimulates lateral root production, thus demonstrating that the inductive signal operative in suspension cultures can be reinterpreted by specific cells later in development and reinitiate meristematic activity. The communication between the precursor cells defined by JIM8 and embryo initials defines an early cell-cell interaction in developing carrot plants. Labelling of flower sections suggests that the same interaction exists between embryo apical and basal cells early in normal development.

AB - In plants, cells differentiate according to their position with relation to their cell neighbours. Monoclonal antibody (MAb) probes to polysaccharide epitopes, present at the surfaces of all plant cells, have defined a family of proteoglycan antigens which signify cellular position. These MAbs have been used to sort the single cells present in carrot somatic cell cultures on the basis of the presence or absence of specific polysaccharide epitopes. This sorting allows embryo initial cells to be cultured among different cell collectives (based on their polysaccharide epitope expression) and thus in altered contextual backgrounds. These experiments have shown that specific populations of embryo initial precursor cells induce and sustain the early development of the embryo initials, revealing that the populations of different cell collectives which are defined by different polysaccharide epitopes (cell-context) serves important regulatory function in early plant development. Somatic embryo initials deprived of the influence of the cell collective — defined by the presence of the polysaccharide epitope recognised by the MAb JIM8 — establish unorganised first divisions and develop as callus. However, in the presence of the JIM8-reactive cell collective, or medium conditioned by the collective, the initials develop into somatic embryos. This demonstrates that the cells defined by the JIM8 polysaccharide epitope are necessary to sustain the meristematic activity which drives the renewed development. Transfer of a cell-wall signal from the JIM8- reactive cells to cellular situations in carrot seedlings in which they would not normally occur (out-of-context signals) stimulates lateral root production, thus demonstrating that the inductive signal operative in suspension cultures can be reinterpreted by specific cells later in development and reinitiate meristematic activity. The communication between the precursor cells defined by JIM8 and embryo initials defines an early cell-cell interaction in developing carrot plants. Labelling of flower sections suggests that the same interaction exists between embryo apical and basal cells early in normal development.

U2 - 10.1098/rstb.1995.0142

DO - 10.1098/rstb.1995.0142

M3 - Article

VL - 350

SP - 87

EP - 93

JO - Philosophical Transactions of the Royal Society B: Biological Sciences

JF - Philosophical Transactions of the Royal Society B: Biological Sciences

SN - 1471-2970

IS - 1331

ER -