Cellulose accessibility determines the rate of enzymatic hydrolysis of steam-pretreated spruce.

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Cellulose accessibility determines the rate of enzymatic hydrolysis of steam-pretreated spruce. / Wiman, Magnus; Dienes, Dora D; Hansen, Mads A T; van der Meulen, Torbjörn; Zacchi, Guido; Lidén, Gunnar.

In: Bioresource Technology, Vol. 126C, 2012, p. 208-215.

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Wiman, Magnus ; Dienes, Dora D ; Hansen, Mads A T ; van der Meulen, Torbjörn ; Zacchi, Guido ; Lidén, Gunnar. / Cellulose accessibility determines the rate of enzymatic hydrolysis of steam-pretreated spruce. In: Bioresource Technology. 2012 ; Vol. 126C. pp. 208-215.

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TY - JOUR

T1 - Cellulose accessibility determines the rate of enzymatic hydrolysis of steam-pretreated spruce.

AU - Wiman, Magnus

AU - Dienes, Dora D

AU - Hansen, Mads A T

AU - van der Meulen, Torbjörn

AU - Zacchi, Guido

AU - Lidén, Gunnar

PY - 2012

Y1 - 2012

N2 - Spruce chips steam-pretreated at various conditions, according to a central composite design, were used for investigating the influence of pretreatment conditions on enzymatic hydrolysis, accounting for the individual effects of pretreatment temperature (194-220°C), time (3-11min) and sulfur dioxide uptake (0.7-2.5%). The materials were analyzed for several surface characteristics, including IR absorption, enzyme adsorption capacity, total surface area, cellulosic surface area, and cellulosic pore sizes. This work showed a clear correlation between rate of enzymatic hydrolysis and specific surface area. Although the lignin content of the particle surface increased at higher pretreatment temperature and residence time, the initial rate of enzymatic hydrolysis increased. Enzyme adsorption measurements and staining methods revealed that the higher rate of hydrolysis of these materials was due to increased accessibility of the cellulose. An accessible cellulose fraction is thus more important than a low surface lignin content for the enzymatic hydrolysis of steam-pretreated spruce.

AB - Spruce chips steam-pretreated at various conditions, according to a central composite design, were used for investigating the influence of pretreatment conditions on enzymatic hydrolysis, accounting for the individual effects of pretreatment temperature (194-220°C), time (3-11min) and sulfur dioxide uptake (0.7-2.5%). The materials were analyzed for several surface characteristics, including IR absorption, enzyme adsorption capacity, total surface area, cellulosic surface area, and cellulosic pore sizes. This work showed a clear correlation between rate of enzymatic hydrolysis and specific surface area. Although the lignin content of the particle surface increased at higher pretreatment temperature and residence time, the initial rate of enzymatic hydrolysis increased. Enzyme adsorption measurements and staining methods revealed that the higher rate of hydrolysis of these materials was due to increased accessibility of the cellulose. An accessible cellulose fraction is thus more important than a low surface lignin content for the enzymatic hydrolysis of steam-pretreated spruce.

U2 - 10.1016/j.biortech.2012.08.082

DO - 10.1016/j.biortech.2012.08.082

M3 - Article

VL - 126C

SP - 208

EP - 215

JO - Bioresource Technology

T2 - Bioresource Technology

JF - Bioresource Technology

SN - 0960-8524

ER -