This chapter summarizes the proteomic tools and strategies used in asthma research. It provides a background to the remodeling process occurring in the lung—in diseases such as asthma and chronic obstructive pulmonary disease (COPD)—as well as a description of the status of the current proteomics techniques. It also describes strategies for the discovery of disease markers that can be used to monitor disease progression/reversibility and strategies for finding molecular features behind the disease. Asthma and COPD are examples of diseases characterized by both inflammation and tissue remodeling. Proteomics is the analysis of the protein complement arising from the genome. Essentially, proteomics requires protein separation and protein identification and in many cases protein quantification. The cornerstones in proteomics are protein/peptide separation by gel electrophoresis and/or chromatography and protein identification by mass spectrometry (MS). Rapid development in different fields—such as separation sciences, MS, and computer-aided software tools—has allowed increasingly difficult tasks being addressed regarding the molecular aspects of the remodeling observed in asthma and COPD. Current state-of-the-art technologies allow the identification, quantification, and classification of thousands of components in a limited time frame.
|Title of host publication||Proteomics and Peptidomics|
|Subtitle of host publication||New Technology Platforms Elucidating Biology|
|Number of pages||22|
|Publication status||Published - 2005 Dec 1|
|Name||Comprehensive Analytical Chemistry|