Chapter 14 Proteomics and remodeling of extracellular matrix in lung diseases

Research output: Chapter in Book/Report/Conference proceedingBook chapter

Abstract

This chapter summarizes the proteomic tools and strategies used in asthma research. It provides a background to the remodeling process occurring in the lung—in diseases such as asthma and chronic obstructive pulmonary disease (COPD)—as well as a description of the status of the current proteomics techniques. It also describes strategies for the discovery of disease markers that can be used to monitor disease progression/reversibility and strategies for finding molecular features behind the disease. Asthma and COPD are examples of diseases characterized by both inflammation and tissue remodeling. Proteomics is the analysis of the protein complement arising from the genome. Essentially, proteomics requires protein separation and protein identification and in many cases protein quantification. The cornerstones in proteomics are protein/peptide separation by gel electrophoresis and/or chromatography and protein identification by mass spectrometry (MS). Rapid development in different fields—such as separation sciences, MS, and computer-aided software tools—has allowed increasingly difficult tasks being addressed regarding the molecular aspects of the remodeling observed in asthma and COPD. Current state-of-the-art technologies allow the identification, quantification, and classification of thousands of components in a limited time frame.

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Subject classification (UKÄ) – MANDATORY

  • Bioinformatics and Systems Biology
Original languageEnglish
Title of host publicationProteomics and Peptidomics
Subtitle of host publicationNew Technology Platforms Elucidating Biology
EditorsV. Marko-Varga
Pages535-556
Number of pages22
Publication statusPublished - 2005 Dec 1
Publication categoryResearch
Peer-reviewedYes

Publication series

NameComprehensive Analytical Chemistry
Volume46
ISSN (Print)0166-526X