Characterization and kinetic analysis of a thermostable GH3 beta-glucosidase from Penicillium brasilianum

Research output: Contribution to journalArticle

Abstract

A GH3 beta-glucosidase (BGL) from Penicillium brasilianum was purified to homogeneity after cultivation on a cellulose and xylan rich medium. The BGL was identified in a genomic library, and it was successfully expressed in Aspergillus oryzae. The BGL had excellent stability at elevated temperatures with no loss in activity after 24 h of incubation at 60A degrees C at pH 4-6, and the BGL was shown to have significantly higher stability at these conditions in comparison to Novozym 188 and to other fungal GH3 BGLs reported in the literature. The BGL had significant lower affinity for cellobiose compared with the artificial substrate para-nitrophenyl-beta-d-glucopyranoside (pNP-Glc) and further, pronounced substrate inhibition using pNP-Glc. Kinetic studies demonstrated the high importance of using cellobiose as substrate and glucose as inhibitor to describe the inhibition kinetics of BGL taking place during cellulose hydrolysis. A novel assay was developed to characterize this glucose inhibition on cellobiose hydrolysis. The assay uses labelled glucose-C-13(6) as inhibitor and subsequent mass spectrometry analysis to quantify the hydrolysis rates.

Details

Authors
  • Kristian B. R. M. Krogh
  • Paul V. Harris
  • Carsten L. Olsen
  • Katja S. Johansen
  • Jesper Hojer-Pedersen
  • Johan Börjesson
  • Lisbeth Olsson
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biological Sciences

Keywords

  • Fungal, Purification, Cellulose hydrolysis, Glucose inhibition, expression, Kinetics
Original languageEnglish
Pages (from-to)143-154
JournalApplied Microbiology and Biotechnology
Volume86
Issue number1
Publication statusPublished - 2010
Publication categoryResearch
Peer-reviewedYes