Characterization of an alternative transcript of the human CREB3L2 gene.

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Characterization of an alternative transcript of the human CREB3L2 gene. / Panagopoulos, Ioannis; Monsef, Nastaran; Collin, Anna; Mertens, Fredrik.

In: Oncology Reports, Vol. 24, No. 5, 2010, p. 1133-1139.

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Panagopoulos, Ioannis ; Monsef, Nastaran ; Collin, Anna ; Mertens, Fredrik. / Characterization of an alternative transcript of the human CREB3L2 gene. In: Oncology Reports. 2010 ; Vol. 24, No. 5. pp. 1133-1139.

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TY - JOUR

T1 - Characterization of an alternative transcript of the human CREB3L2 gene.

AU - Panagopoulos, Ioannis

AU - Monsef, Nastaran

AU - Collin, Anna

AU - Mertens, Fredrik

PY - 2010

Y1 - 2010

N2 - CREB3L2, a member of the CREB3 family of transcription factors, spans >120 kbp and is composed of 12 exons. We characterized a widely expressed transcript of CREB3L2 generated by an intronic polyadenylation site in intron 4 of the gene. It could be translated to a CREB3L2 variant which is localized both in the nucleus and the endoplasmatic reticulum. The protein retains the N-terminal transactivation domain but lacks the DNA-binding domain, the transmembrane domain and the C-terminal part. Experiments using a GAL4 DNA-binding domain fusion model showed that the transcript is a transactivator but it cannot exert its function through the CRE and ATF6 binding sites and has little effect on the GRP78 promoter. Whether this transcript has a cellular function or is targeted for degradation by nonsense-mediated RNA decay system of RNA surveillance is currently unknown.

AB - CREB3L2, a member of the CREB3 family of transcription factors, spans >120 kbp and is composed of 12 exons. We characterized a widely expressed transcript of CREB3L2 generated by an intronic polyadenylation site in intron 4 of the gene. It could be translated to a CREB3L2 variant which is localized both in the nucleus and the endoplasmatic reticulum. The protein retains the N-terminal transactivation domain but lacks the DNA-binding domain, the transmembrane domain and the C-terminal part. Experiments using a GAL4 DNA-binding domain fusion model showed that the transcript is a transactivator but it cannot exert its function through the CRE and ATF6 binding sites and has little effect on the GRP78 promoter. Whether this transcript has a cellular function or is targeted for degradation by nonsense-mediated RNA decay system of RNA surveillance is currently unknown.

U2 - 10.3892/or_00000964

DO - 10.3892/or_00000964

M3 - Article

VL - 24

SP - 1133

EP - 1139

JO - Oncology Reports

JF - Oncology Reports

SN - 1791-2431

IS - 5

ER -