Characterization of major enzymes and genes involved in flavonoid and proanthocyanidin biosynthesis during fruit development in strawberry (Fragaria x ananassa)

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Characterization of major enzymes and genes involved in flavonoid and proanthocyanidin biosynthesis during fruit development in strawberry (Fragaria x ananassa). / Almeida, Joao; D'Amico, Eleonora; Preuss, Anja; Carbone, Fabrizio; de Vos, C. H. Ric; Deiml, Bettina; Mourgues, Fabienne; Perrotta, Gaetano; Fischer, Thilo C.; Bovy, Arnaud G.; Martens, Stefan; Rosati, Carlo.

In: Archives of Biochemistry and Biophysics, Vol. 465, No. 1, 2007, p. 61-71.

Research output: Contribution to journalArticle

Harvard

Almeida, J, D'Amico, E, Preuss, A, Carbone, F, de Vos, CHR, Deiml, B, Mourgues, F, Perrotta, G, Fischer, TC, Bovy, AG, Martens, S & Rosati, C 2007, 'Characterization of major enzymes and genes involved in flavonoid and proanthocyanidin biosynthesis during fruit development in strawberry (Fragaria x ananassa)', Archives of Biochemistry and Biophysics, vol. 465, no. 1, pp. 61-71. https://doi.org/10.1016/j.abb.2007.04.040

APA

Almeida, J., D'Amico, E., Preuss, A., Carbone, F., de Vos, C. H. R., Deiml, B., Mourgues, F., Perrotta, G., Fischer, T. C., Bovy, A. G., Martens, S., & Rosati, C. (2007). Characterization of major enzymes and genes involved in flavonoid and proanthocyanidin biosynthesis during fruit development in strawberry (Fragaria x ananassa). Archives of Biochemistry and Biophysics, 465(1), 61-71. https://doi.org/10.1016/j.abb.2007.04.040

CBE

MLA

Vancouver

Author

Almeida, Joao ; D'Amico, Eleonora ; Preuss, Anja ; Carbone, Fabrizio ; de Vos, C. H. Ric ; Deiml, Bettina ; Mourgues, Fabienne ; Perrotta, Gaetano ; Fischer, Thilo C. ; Bovy, Arnaud G. ; Martens, Stefan ; Rosati, Carlo. / Characterization of major enzymes and genes involved in flavonoid and proanthocyanidin biosynthesis during fruit development in strawberry (Fragaria x ananassa). In: Archives of Biochemistry and Biophysics. 2007 ; Vol. 465, No. 1. pp. 61-71.

RIS

TY - JOUR

T1 - Characterization of major enzymes and genes involved in flavonoid and proanthocyanidin biosynthesis during fruit development in strawberry (Fragaria x ananassa)

AU - Almeida, Joao

AU - D'Amico, Eleonora

AU - Preuss, Anja

AU - Carbone, Fabrizio

AU - de Vos, C. H. Ric

AU - Deiml, Bettina

AU - Mourgues, Fabienne

AU - Perrotta, Gaetano

AU - Fischer, Thilo C.

AU - Bovy, Arnaud G.

AU - Martens, Stefan

AU - Rosati, Carlo

PY - 2007

Y1 - 2007

N2 - The biosynthesis of flavonoids and proanthocyanidins was studied in cultivated strawberry (Fragaria x ananassa) by combining biochemical and molecular approaches. Chemical analyses showed that ripe strawberries accumulate high amounts of pelargonidin-derived anthocyanins, and a larger pool of 3',4'-hydroxylated proanthocyanidins. Activities and properties of major recombinant enzymes were demonstrated by means of in vitro assays, with special emphasis on specificity for the biologically relevant 4'- and 3',4'-hydroxylated compounds. Only leucoanthocyanidin reductase showed a strict specificity for the 3',4'-hydroxylated leucocyanidin, while other enzymes accepted either hydroxylated substrate with different relative activity rates. The structure of late flavonoid pathway genes, leading to the synthesis of major compounds in ripe fruits, was elucidated. Complex developmental and spatial expression patterns were shown for phenylpropanoid and flavonoid genes in fruits throughout ripening as well as in leaves, petals and roots. Presented results elucidate key steps in the biosynthesis of strawberry flavonoid end products. (c) 2007 Elsevier Inc. All rights reserved.

AB - The biosynthesis of flavonoids and proanthocyanidins was studied in cultivated strawberry (Fragaria x ananassa) by combining biochemical and molecular approaches. Chemical analyses showed that ripe strawberries accumulate high amounts of pelargonidin-derived anthocyanins, and a larger pool of 3',4'-hydroxylated proanthocyanidins. Activities and properties of major recombinant enzymes were demonstrated by means of in vitro assays, with special emphasis on specificity for the biologically relevant 4'- and 3',4'-hydroxylated compounds. Only leucoanthocyanidin reductase showed a strict specificity for the 3',4'-hydroxylated leucocyanidin, while other enzymes accepted either hydroxylated substrate with different relative activity rates. The structure of late flavonoid pathway genes, leading to the synthesis of major compounds in ripe fruits, was elucidated. Complex developmental and spatial expression patterns were shown for phenylpropanoid and flavonoid genes in fruits throughout ripening as well as in leaves, petals and roots. Presented results elucidate key steps in the biosynthesis of strawberry flavonoid end products. (c) 2007 Elsevier Inc. All rights reserved.

KW - developmental gene expression

KW - proanthocyanidins

KW - recombinant enzyme activity

KW - strawberry

KW - preference

KW - substrate

KW - flavonoids

KW - fruit ripening

U2 - 10.1016/j.abb.2007.04.040

DO - 10.1016/j.abb.2007.04.040

M3 - Article

VL - 465

SP - 61

EP - 71

JO - Archives of Biochemistry and Biophysics

JF - Archives of Biochemistry and Biophysics

SN - 0003-9861

IS - 1

ER -