Characterization of molecularly imprinted polymer nanoparticles by photon correlation spectroscopy

Research output: Contribution to journalArticle

Abstract

We follow template-binding induced aggregation of nanoparticles enantioselectively imprinted against (S)-propranolol, and the non-imprinted ones, using photon correlation spectroscopy (dynamic light scattering). The method requires no separation steps. We have characterized binding of (R,S)-propranolol to the imprinted polymers and determined the degree of non-specificity by comparing the specific binding with the results obtained using non-imprinted nanoparticles. Using (S)-propranolol as a template for binding to (S)-imprinted nanoparticle, and (R)-propranolol as a non-specific control, we have determined range of concentrations where chiral recognition can be observed. By studying aggregation induced by three analytes related to propranolol, atenolol, betaxolol, and 1-amino-3-(naphthalen-1-yloxy)propan-2-ol, we were able to determine which parts of the template are involved in the specific binding, discuss several details of specific adsorption, and the structure of the imprinted site. Copyright (c) 2014 John Wiley & Sons, Ltd.

Details

Authors
  • Bjoern Malm
  • Keiichi Yoshimatsu
  • Lei Ye
  • Anatol Krozer
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology

Keywords

  • molecularly imprinted polymers, Brownian motion, chiral selectivity, aggregation kinetics, colloidal suspension
Original languageEnglish
Pages (from-to)714-721
JournalJournal of Molecular Recognition
Volume27
Issue number12
Publication statusPublished - 2014
Publication categoryResearch
Peer-reviewedYes