Characterization of multimodal hydrophobic interaction chromatography media useful for isolation of green fluorescent proteins with small structural differences.

Research output: Contribution to journalArticle


Hydrophobic interaction chromatography (HIC) has been developed as a powerful technique for separating and purifying proteins. In this study, we have characterized the ability of new multimodal pH-HIC media to resolve proteins with only small differences in their primary structures. This was done by determining the retention times of different green fluorescent protein (GFP) mutants prepared from Escherichia coli extracts. The mutants, modified with single or double hydrophobic amino acid substitutions in two positions, N212 and T230, could be resolved successfully, up to 2.1 column volumes in retention difference for single substitutions and 2.6 column volumes for double substitutions, at two pH and on two media with varying ligand density. The retention times also correlated well with calculated theoretical retentions (R(2) = 0.91) using a hydrophobic descriptor. This medium can therefore be very useful in a final polishing step during purification and the protein library prepared represents a good screening set in validating and characterizing new future media due to the accessible, but yet, extremely small differences in protein structure. Copyright (c) 2008 John Wiley & Sons, Ltd.


  • Kristian Becker
  • Elisabeth Hallgren
  • Enrique Carredano
  • Ronnie Palmgren
  • Leif Bülow
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biochemistry and Molecular Biology


  • green fluorescent protein, hydrophobic interaction chromatography, tyrosine tag, pH-responsive, polymer ligand
Original languageEnglish
Pages (from-to)104-109
JournalJournal of Molecular Recognition
Issue number2
Publication statusPublished - 2009
Publication categoryResearch

Bibliographic note

Published Online: 24 Jul 2008