Characterization of the biochemical and biophysical properties of the Saccharomyces cerevisiae phosphate transporter Pho89

Research output: Contribution to journalArticle

Abstract

In Saccharomyces cerevisiae, Pho89 mediates a cation-dependent transport of Pi across the plasma membrane. This integral membrane protein belongs to the Inorganic Phosphate Transporter (PiT) family, a group that includes the mammalian Na(+)/Pi cotransporters Pit1 and Pit2. Here we report that the Pichia pastoris expressed recombinant Pho89 was purified in the presence of Foscholine-12 and functionally reconstituted into proteoliposomes with a similar substrate specificity as observed in an intact cell system. The alpha-helical content of the Pho89 protein was estimated to 44%. EPR analysis showed that purified Pho89 protein undergoes conformational change upon addition of substrate.

Details

Authors
Organisations
External organisations
  • Linnaeus University
  • University of California, Davis
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biological Sciences

Keywords

  • Pho89, Pichia pastoris, Oligomer, Reconstitution, Phosphate transport, Circular dichroism
Original languageEnglish
Pages (from-to)551-556
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume436
Issue number3
Publication statusPublished - 2013 Jul 5
Publication categoryResearch
Peer-reviewedYes