Characterization of the outer membrane protein profile from disease-related Helicobacter pylori isolates by subcellular fractionation and nano-LC FT-ICR MS analysis

Research output: Contribution to journalArticle


Because of the important role of membrane proteins in adhesion, invasion, and intracellular survival of pathogens in the host, membrane proteins are of potential interest in the search for drug targets or biomarkers. We have established a mass spectrometry-based method that allows characterization of the outer membrane protein (OMP) profile of clinical isolates from of the human gastric pathogen Helicobacter pylori. Subcellular fractionation and one-dimensional gel electrophoresis (1D-GE) analysis was combined with nano-liquid chromatography Fourier transform-ion cyclotron resonance mass spectrometry (nano-LC FT-ICR MS) and tandem mass spectrometry (MS/MS) analysis of fifteen H. pylori strains associated either with duodenal ulcers, gastric cancer, or isolated from asymptomatic H. pylori infected carriers. Over 60 unique membrane or membrane-associated proteins, including 30 of the 33 theoretically predicted OMPs, were identified from the strains. Several membrane proteins, including Omp11 and BabA, were found to be expressed by all strains. In the search for clinical markers we found that Omp26 was expressed by all disease-related strains but was only present in one out of five strains from asymptomatic carriers, which makes Omp26 a potential target for further investigation in the search for proteins unique to disease-related H. pylori strains. In addition, presence of Omp30 and absence of Omp6 seemed to be associated with H. pylori strains causing duodenal ulcer.


  • Elisabet Carlsohn
  • Johanna Nyström
  • Hasse Karlsson
  • Ann-Mari Svennerholm
  • Carol L Nilsson
External organisations
  • Florida State University
Research areas and keywords


  • Amino Acid Sequence, Bacterial Outer Membrane Proteins, Bacterial Proteins, Cyclotrons, Helicobacter Infections, Helicobacter pylori, Humans, Mass Spectrometry, Membrane Proteins, Molecular Sequence Data, Nanotechnology, Peptide Fragments, Phylogeny, Proteomics, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.
Original languageEnglish
Pages (from-to)3197-204
Number of pages8
JournalJournal of Proteome Research
Issue number11
Publication statusPublished - 2006 Nov
Publication categoryResearch
Externally publishedYes