Chlorhexidine for prevention of neonatal colonization with group B streptococci. I. In vitro effect of chlorhexidine on group B streptococci

Research output: Contribution to journalArticle

Abstract

Forty-three strains of group B streptococci (GBS) of types Ia, Ib, II and III were tested for susceptibility to chlorhexidine in concentrations ranging from 256 to 0.25 mg/l using the agar and tube dilution methods. The strains showed minimum inhibitory concentration (MIC) values ranging from 0.5 to 1 mg/l. Serum added to the test medium (50%) increased the MIC values to 4-8 mg/l, while amniotic fluid (50%) had almost no effect, increasing the values to 1-2 mg/l. The minimum bactericidal concentration (MBC) ranged from 1 to 5 mg/l. The killing kinetics were related to the concentration of chlorhexidine and the length of exposure. For example, at a concentration of 63 mg/l, 7 h were required for a bactericidal effect in broth, as compared to 1 h at 500 mg/l chlorhexidine. 200 mg/l chlordexidine had no effect on the adherence of two GBS strains to vaginal epithelial cells, and no effect on the phagocytosis of GBS with mouse peritoneal macrophages.

Details

Authors
  • Karen Kvist Christensen
  • Poul Christensen
  • Anna-Karin Dykes
  • Gunnar Kahlmeter
  • Daya N Kurl
  • Viveka Linden
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Obstetrics, Gynecology and Reproductive Medicine

Keywords

  • group B streptococci, chlorhexidine, minimal inhibitory concentration, minimal bactericidal concentration, adherence, phagocytosis
Original languageEnglish
Pages (from-to)157-165
JournalEuropean Journal of Obstetrics, Gynecology, and Reproductive Biology
Volume16
Issue number3
Publication statusPublished - 1983
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division of Nursing (Closed 2012) (013065000), Department of Obstetrics and Gynaecology (Lund) (013018000), Division of Medical Microbiology (013250400)