Chondroitin sulfate perlecan enhances collagen fibril formation - Implications for perlecan chondrodysplasias

Research output: Contribution to journalArticle

Abstract

Inactivation of the perlecan gene leads to perinatal lethal chondrodysplasia. The similarity to the phenotypes of the Col2A1 knock-out and the disproportionate micromelia mutation suggests perlecan involvement in cartilage collagen matrix assembly. We now present a mechanism for the defect in collagen type II fibril assembly by perlecan-null chondrocytes. Cartilage perlecan is a heparin sulfate or a mixed heparan sulfate/ chondroitin sulfate proteoglycan. The latter form binds collagen and accelerates fibril formation in vitro, with more defined fibril morphology and increased fibril diameters produced in the presence of perlecan. Interestingly, the enhancement of collagen fibril formation is independent on the core protein and is mimicked by chondroitin sulfate E but neither by chondroitin sulfate D nor dextran sulfate. Furthermore, perlecan chondroitin sulfate contains the 4,6-disulfated disaccharides typical for chondroitin sulfate E. Indeed, purified glycosaminoglycans from perlecan-enriched fractions of cartilage extracts contain elevated levels of 4,6-disulfated chondroitin sulfate disaccharides and enhance collagen fibril formation. The effect on collagen assembly is proportional to the content of the 4,6- disulfated disaccharide in the different cartilage extracts, with growth plate cartilage glycosaminoglycan being the most efficient enhancer. These findings demonstrate a role for perlecan chondroitin sulfate side chains in cartilage extracellular matrix assembly and provide an explanation for the perlecan-null chondrodysplasia.

Details

Authors
  • Alexander Kvist
  • Anna Johnson
  • Matthias Mörgelin
  • Erika Gustafsson
  • Eva Bengtsson
  • Karin Lindblom
  • Attila Aszodi
  • Reinhard Faessler
  • Takako Sasaki
  • Rupert Timpl
  • Anders Aspberg
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Basic Medicine
  • Other Clinical Medicine
Original languageEnglish
Pages (from-to)33127-33139
JournalJournal of Biological Chemistry
Volume281
Issue number44
Publication statusPublished - 2006
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Department of Clinical Sciences, Lund (013230000), Department of Experimental Medical Science (013210000), Connective Tissue Biology (013230151), Division of Infection Medicine (BMC) (013024020), Pathology, (Lund) (013030000), Experimental Cardiovascular Research Unit (013242110)