Chromosomal integration and expression of green fluorescent protein in Zymomonas mobilis

Research output: Contribution to journalArticle

Abstract

An integrative vector was constructed to allow expression of heterologous proteins into the adhB locus of Zymomonas mobilis. As a reporter gene, the ORF of a bright variant of green fluorescent protein from Aequorea victoria (GFPuv) was fused to the adhB strong promoter from Z. mobilis by using a two-step PCR strategy. Z. mobilis recombinant strains that were stably marked by precise gene replacement at adhB locus with a single chromosomal copy of gfpuv. Protein expression was confirmed by fluorescence microscopy and measured by fluorescence spectroscopy, showing high expression levels (12 to 30 times higher than those obtained in E. coli) without affecting the host growth.

Details

Authors
  • Osvaldo Delgado
  • MA Martinez
  • CM Abate
  • F Sineriz
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Industrial Biotechnology

Keywords

  • PCR, Zymomonas mobilis, gfpuv, alcohol dehydrogenase, expression vector
Original languageEnglish
Pages (from-to)1285-1290
JournalBiotechnology Letters
Volume24
Issue number15
Publication statusPublished - 2002
Publication categoryResearch
Peer-reviewedYes