Circadian rhythms persist without transcription in a eukaryote

Research output: Contribution to journalArticle

Abstract

Circadian rhythms are ubiquitous in eukaryotes, and coordinate numerous aspects of behaviour, physiology and metabolism, from sleep/wake cycles in mammals to growth and photosynthesis in plants. This daily timekeeping is thought to be driven by transcriptionaltranslational feedback loops, whereby rhythmic expression of clock- gene products regulates the expression of associated genes in approximately 24-hour cycles. The specific transcriptional components differ between phylogenetic kingdoms. The unicellular pico-eukaryotic alga Ostreococcus tauri possesses a naturally minimized clock, which includes many features that are shared with plants, such as a central negative feedback loop that involves the morning-expressed CCA1 and evening-expressed TOC1 genes. Given that recent observations in animals and plants have revealed prominent post-translational contributions to timekeeping, a reappraisal of the transcriptional contribution to oscillator function is overdue. Here we show that non-transcriptional mechanisms are sufficient to sustain circadian timekeeping in the eukaryotic lineage, although they normally function in conjunction with transcriptional components. We identify oxidation of peroxiredoxin proteins as a transcription-independent rhythmic biomarker, which is also rhythmic in mammals. Moreover we show that pharmacological modulators of the mammalian clock mechanism have the same effects on rhythms in Ostreococcus. Post-translational mechanisms, and at least one rhythmic marker, seem to be better conserved than transcriptional clock regulators. It is plausible that the oldest oscillator components are non-transcriptional in nature, as in cyanobacteria, and are conserved across kingdoms.

Details

Authors
  • John S. O'Neill
  • Gerben Van Ooijen
  • Laura E. Dixon
  • Carl Troein
  • Florence Corellou
  • François-Yves Bouget
  • Akhilesh B. Reddy
  • Andrew J. Millar
External organisations
  • Centre for Systems Biology at Edinburgh
  • University of Cambridge
  • University of Edinburgh
  • UPMCUniv Paris 06
  • Laboratoire d'Océanographie Microbienne (LOMIC)
  • Versailles Saint-Quentin-en-Yvelines University
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Genetics
  • Plant Biotechnology
Original languageEnglish
Pages (from-to)554-558
Number of pages5
JournalNature
Volume469
Issue number7331
Publication statusPublished - 2011 Jan 27
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes