Clinical Complement Analysis—An Overview

Research output: Contribution to journalReview article

Abstract

The complement system plays an important role in varying types of disease, ranging from inflammatory and autoimmune disorders to immune deficiency states. In addition, new settings have emerged where complement analysis is of interest to monitor complement-directed therapy and aid identification of transplant complications. Therefore, it is critical that clinical laboratories offer optimized and timely complement analysis. This review presents a comprehensive overview of the most important complement analysis methods that are currently used. It also points to some areas within complement diagnostics where development is needed, for example, regarding certain analytes for which practical methods suitable for the routine laboratory are lacking. Furthermore, it contains a more detailed discussion on complement autoantibody assessment. The list of analyses providing clinically valuable information includes analysis of complement function, quantification of individual complement components and complement activation fragments, identification of autoantibodies to complement, as well as genetic complement analyses. There is still a shortage of commercially available methods suitable for high-throughput screening of complement deficiency and for assessment of complement activation, but development is under way. There is also ongoing work within the complement community to improve standardization of measurements, and recently, an extensive quality assurance program has been initiated.

Details

Authors
Organisations
External organisations
  • Region Skåne
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Hematology
  • Medical Laboratory and Measurements Technologies

Keywords

  • Clinical complement analysis, Complement activation/consumption, Complement deficiency, Complement diagnostics, Complement function
Original languageEnglish
Pages (from-to)207-216
JournalTransfusion Medicine Reviews
Volume33
Issue number4
Early online date2019 Oct 18
Publication statusPublished - 2019
Publication categoryResearch
Peer-reviewedYes