Clonal chromosome aberrations are present in vivo in synovia and osteophytes from patients with osteoarthritis

Research output: Contribution to journalArticle

Abstract

We have previously reported recurrent clonal chromosomal aberrations in synovia, osteophytes and articular cartilage from patients with osteoarthritis (OA). In particular, gain of chromosomes 5 and 7 was found to be strongly associated with OA. In order to exclude the possibility of in vitro artefacts, we studied three to four parallel, independent cultures from ten samples of synovia and three samples of osteophytes from ten women with primary OA. In all, 40 cultures were cytogenetically analysed, 39 of which had clonal chromosomal aberrations. The most common aberrations were +7 and +5 which were found in 38 and 12 cultures, respectively. There were striking karyotype similarities among the parallel cultures from each case. Out of a total of 83 clones, only 11 were unique for one culture, 7 from synovia and 4 from osteophytes. The genetic homogeneity among different cultures from the same patients excludes the possibility of in vitro artefacts and indicates a widespread distribution of the cytogenetically aberrant clones in vivo.

Details

Authors
Organisations
External organisations
  • Skåne University Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Rheumatology and Autoimmunity

Keywords

  • Aged, Aged, 80 and over, Bone and Bones/pathology, Chromosome Aberrations, Chromosomes, Human, Pair 5, Chromosomes, Human, Pair 7, Clone Cells, Cytogenetics, Female, Humans, Karyotyping, Knee Joint/pathology, Osteoarthritis/genetics, Osteoblasts/pathology, Synovial Membrane/pathology
Original languageEnglish
Pages (from-to)295-8
Number of pages4
JournalHuman Genetics
Volume101
Issue number3
Publication statusPublished - 1997 Dec
Publication categoryResearch
Peer-reviewedYes