Coevolution with bacteria drives the evolution of aerobic fermentation in Lachancea kluyveri

Research output: Contribution to journalArticle

Abstract

The Crabtree positive yeasts, such as Saccharomyces cerevisiae, prefer fermentation to respiration, even under fully aerobic conditions. The selective pressures that drove the evolution of this trait remain controversial because of the low ATP yield of fermentation compared to respiration. Here we propagate experimental populations of the weak-Crabtree yeast Lachancea kluyveri, in competitive co-culture with bacteria. We find that L. kluyveri adapts by producing quantities of ethanol lethal to bacteria and evolves several of the defining characteristics of Crabtree positive yeasts. We use precise quantitative analysis to show that the rate advantage of fermentation over aerobic respiration is insufficient to provide an overall growth advantage. Thus, the rapid consumption of glucose and the utilization of ethanol are essential for the success of the aerobic fermentation strategy. These results corroborate that selection derived from competition with bacteria could have provided the impetus for the evolution of the Crabtree positive trait.

Details

Authors
  • Nerve Zhou
  • Krishna B S Swamy
  • Jun Yi Leu
  • Michael J. McDonald
  • Silvia Galafassi
  • Concetta Compagno
  • Jure Piskur
Organisations
External organisations
  • Monash University
  • University of Milan
  • Institute of Molecular Biology, Academia Sinica
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell Biology
  • Microbiology
Original languageEnglish
Article numbere0173318
JournalPLoS ONE
Volume12
Issue number3
Publication statusPublished - 2017 Mar 1
Publication categoryResearch
Peer-reviewedYes