Coexpression of neurotrophins and their receptors in neurons of the central nervous system

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T1 - Coexpression of neurotrophins and their receptors in neurons of the central nervous system

AU - Kokaia, Z

AU - Bengzon, J

AU - Metsis, M

AU - Kokaia, M

AU - Persson, H

AU - Lindvall, O

PY - 1993/7/15

Y1 - 1993/7/15

N2 - Nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) are neuronal survival molecules which utilize the Trk family of tyrosine kinase receptors. Using double-label in situ hybridization, we demonstrate that mRNAs for BDNF and its high-affinity receptor TrkB are coexpressed in hippocampal and cortical neurons. Also, a large number of neurons in these areas coexpress NGF and BDNF mRNAs. Epileptic seizures lead to increased levels of both BDNF/TrkB and NGF/BDNF mRNAs in double-labeled cells. Our results show that individual neurons of the central nervous system can coexpress neurotrophins and their receptors and produce two neurotrophic factors. These factors could support neuronal survival after brain insults, not only via retrograde transport but also through autocrine mechanisms.

AB - Nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) are neuronal survival molecules which utilize the Trk family of tyrosine kinase receptors. Using double-label in situ hybridization, we demonstrate that mRNAs for BDNF and its high-affinity receptor TrkB are coexpressed in hippocampal and cortical neurons. Also, a large number of neurons in these areas coexpress NGF and BDNF mRNAs. Epileptic seizures lead to increased levels of both BDNF/TrkB and NGF/BDNF mRNAs in double-labeled cells. Our results show that individual neurons of the central nervous system can coexpress neurotrophins and their receptors and produce two neurotrophic factors. These factors could support neuronal survival after brain insults, not only via retrograde transport but also through autocrine mechanisms.

KW - Animals

KW - Brain/cytology

KW - Brain Chemistry

KW - Brain-Derived Neurotrophic Factor

KW - Cerebral Cortex/chemistry

KW - Hippocampus/chemistry

KW - In Situ Hybridization

KW - Male

KW - Membrane Proteins/isolation & purification

KW - Nerve Growth Factors/isolation & purification

KW - Nerve Tissue Proteins/isolation & purification

KW - Neurons/chemistry

KW - RNA, Messenger/isolation & purification

KW - Rats

KW - Rats, Sprague-Dawley

KW - Receptor, Ciliary Neurotrophic Factor

KW - Seizures

U2 - 10.1073/pnas.90.14.6711

DO - 10.1073/pnas.90.14.6711

M3 - Article

VL - 90

SP - 6711

EP - 6715

JO - Proceedings of the National Academy of Sciences

JF - Proceedings of the National Academy of Sciences

SN - 1091-6490

IS - 14

ER -