Comparative genome- and proteome analysis of cerebral cortex from MK-801-treated rats

Research output: Contribution to journalArticle

Abstract

cDNA microarrays and two-dimensional gel-electrophoresis in combination with mass spectrometry, were used to screen alterations in mRNA and protein levels, respectively, in cerebral cortex of MK-801-treated rats. The rats were divided in two groups; group 1 (short-term treated) and group 2 (long-term treated). In group 1, four genes were up-regulated and five down-regulated. In group 2, seven genes were up-regulated and six down-regulated. In group 1, the levels of one protein was increased and eight proteins reduced. In group 2, the levels of two proteins were increased and four proteins reduced. Several of the altered genes (casein kinase 2, glutamic acid decarboxylase, synaptotagmin, gamma aminobutyric acid [GABA] transporter, creatine kinase, and cytochrome c oxidase) and proteins (superoxide dismutase, hsp 60, hsp 72 and gamma-enolase) have previously been connected to schizophrenia. Alterations of the genes (microglobulin, c-jun proto-oncogene, 40S ribosomal protein S19, adenosine diphosphate (ADP)-ribosylation factors, platelet-derived growth factor, fructose-bisphophate aldolase A, and myelin proteolipid) and the proteins (stathmin, H+-transp. Adenosine triphosphate (ATP) synthase, pyruvate dehydrogenase, beta-actin and alpha-enolase), have not, to our knowledge, earlier been implicated in schizophrenia pathology. Overall, these results with a combined approach of genomics and proteomics add to the validity of subchronic N-methyl-D-aspartate (NMDA)-receptor antagonist treatment as an animal model of schizophrenia.

Details

Authors
  • Linda Paulson
  • Peter Martin
  • Anders Persson
  • Carol L Nilsson
  • Elisabeth Ljung
  • Ann Westman-Brinkmalm
  • Peter S Eriksson
  • Kaj Blennow
  • Pia Davidsson
External organisations
  • University of Gothenburg
Research areas and keywords

Keywords

  • Animals, Cerebral Cortex, Dizocilpine Maleate, Gene Expression Regulation, Genome, Oligonucleotide Array Sequence Analysis, Proteome, RNA, Messenger, Rats, Rats, Sprague-Dawley, Schizophrenia, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
Original languageEnglish
Pages (from-to)526-33
Number of pages8
JournalJournal of Neuroscience Research
Volume71
Issue number4
Publication statusPublished - 2003 Feb 15
Publication categoryResearch
Peer-reviewedYes
Externally publishedYes