Comparison of Radioimmunoprecipitation With Luciferase Immunoprecipitation for Autoantibodies to GAD65 and IA-2 beta

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Bibtex

@article{e6a55e8ec6ea40dfa3d9dae69d8524bf,
title = "Comparison of Radioimmunoprecipitation With Luciferase Immunoprecipitation for Autoantibodies to GAD65 and IA-2 beta",
abstract = "OBJECTIVE - To compare the sensitivity and specificity of luciferase immunoprecipitation (LIPS) with radioimmunoprecipitation (RIP) for the measurement of autoantibodies to the type 1 diabetes autoantigens glutamic acid decarboxylase 65 (GAD65) and insulinoma-associated protein (IA)-2 beta. RESEARCH DESIGN AND METHODS - Sera from 49 type 1 diabetic patients and 100 nondiabetic control subjects from Diabetes Antibody Standardization Program 2007 were used to screen for autoantibodies to GAD65. An additional 200 type 1 diabetic patients and 200 nondiabetic control subjects were used to validate the GAD65 results and screen for autoantibodies to IA-2 beta. RESULTS - LIPS showed equal sensitivity and specificity to RIP for detecting autoantibodies to GAD65 and IA-2 beta. Receiver-operating characteristic analysis revealed that the detection of autoantibodies to GAD65 and IA-2 beta by LIPS and RIP were not statistically different. CONCLUSIONS - The LIPS assay does not require the use of radioisotopes or in vitro transcription/translation and is a practical alternative at the clinical level for the RIP assay.",
author = "Burbelo, {Peter D.} and Hiroki Hirai and Issa, {Alexandra T.} and Albert Kingman and {\AA}ke Lernmark and Sten Ivarsson and Notkins, {Abner L.} and Iadarola, {Michael J.}",
year = "2010",
doi = "10.2337/dc09-1938",
language = "English",
volume = "33",
pages = "754--756",
journal = "Diabetes Care",
issn = "1935-5548",
publisher = "American Diabetes Association",
number = "4",

}