Comparison of Radioimmunoprecipitation With Luciferase Immunoprecipitation for Autoantibodies to GAD65 and IA-2 beta

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Comparison of Radioimmunoprecipitation With Luciferase Immunoprecipitation for Autoantibodies to GAD65 and IA-2 beta. / Burbelo, Peter D.; Hirai, Hiroki; Issa, Alexandra T.; Kingman, Albert; Lernmark, Åke; Ivarsson, Sten; Notkins, Abner L.; Iadarola, Michael J.

In: Diabetes Care, Vol. 33, No. 4, 2010, p. 754-756.

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Burbelo, Peter D. ; Hirai, Hiroki ; Issa, Alexandra T. ; Kingman, Albert ; Lernmark, Åke ; Ivarsson, Sten ; Notkins, Abner L. ; Iadarola, Michael J. / Comparison of Radioimmunoprecipitation With Luciferase Immunoprecipitation for Autoantibodies to GAD65 and IA-2 beta. In: Diabetes Care. 2010 ; Vol. 33, No. 4. pp. 754-756.

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TY - JOUR

T1 - Comparison of Radioimmunoprecipitation With Luciferase Immunoprecipitation for Autoantibodies to GAD65 and IA-2 beta

AU - Burbelo, Peter D.

AU - Hirai, Hiroki

AU - Issa, Alexandra T.

AU - Kingman, Albert

AU - Lernmark, Åke

AU - Ivarsson, Sten

AU - Notkins, Abner L.

AU - Iadarola, Michael J.

PY - 2010

Y1 - 2010

N2 - OBJECTIVE - To compare the sensitivity and specificity of luciferase immunoprecipitation (LIPS) with radioimmunoprecipitation (RIP) for the measurement of autoantibodies to the type 1 diabetes autoantigens glutamic acid decarboxylase 65 (GAD65) and insulinoma-associated protein (IA)-2 beta. RESEARCH DESIGN AND METHODS - Sera from 49 type 1 diabetic patients and 100 nondiabetic control subjects from Diabetes Antibody Standardization Program 2007 were used to screen for autoantibodies to GAD65. An additional 200 type 1 diabetic patients and 200 nondiabetic control subjects were used to validate the GAD65 results and screen for autoantibodies to IA-2 beta. RESULTS - LIPS showed equal sensitivity and specificity to RIP for detecting autoantibodies to GAD65 and IA-2 beta. Receiver-operating characteristic analysis revealed that the detection of autoantibodies to GAD65 and IA-2 beta by LIPS and RIP were not statistically different. CONCLUSIONS - The LIPS assay does not require the use of radioisotopes or in vitro transcription/translation and is a practical alternative at the clinical level for the RIP assay.

AB - OBJECTIVE - To compare the sensitivity and specificity of luciferase immunoprecipitation (LIPS) with radioimmunoprecipitation (RIP) for the measurement of autoantibodies to the type 1 diabetes autoantigens glutamic acid decarboxylase 65 (GAD65) and insulinoma-associated protein (IA)-2 beta. RESEARCH DESIGN AND METHODS - Sera from 49 type 1 diabetic patients and 100 nondiabetic control subjects from Diabetes Antibody Standardization Program 2007 were used to screen for autoantibodies to GAD65. An additional 200 type 1 diabetic patients and 200 nondiabetic control subjects were used to validate the GAD65 results and screen for autoantibodies to IA-2 beta. RESULTS - LIPS showed equal sensitivity and specificity to RIP for detecting autoantibodies to GAD65 and IA-2 beta. Receiver-operating characteristic analysis revealed that the detection of autoantibodies to GAD65 and IA-2 beta by LIPS and RIP were not statistically different. CONCLUSIONS - The LIPS assay does not require the use of radioisotopes or in vitro transcription/translation and is a practical alternative at the clinical level for the RIP assay.

U2 - 10.2337/dc09-1938

DO - 10.2337/dc09-1938

M3 - Article

C2 - 20086252

VL - 33

SP - 754

EP - 756

JO - Diabetes Care

JF - Diabetes Care

SN - 1935-5548

IS - 4

ER -