Complement in Autoimmunity - the importance of clearing waste

Research output: ThesisDoctoral Thesis (compilation)

Abstract

Systemic lupus erythematosus (SLE) is an autoimmune disease where improperly cleared apoptotic cells and neutrophil extracellular traps (NETs) induce an autoimmune response. Complement is crucial to prevent SLE by tailoring immune responses and opsonizing dead cells but may also induce inflammation and tissue damage once the disease is initiated.
Complement C1q binds to apoptotic cells and ensures rapid and tolerogenic clearance by phagocytes. C1q can also activate complement. To avoid excessive activation, binding of the complement inhibitor factor H to the apoptotic cells is crucial. In this thesis we have discovered that factor H as well as C1q bind to the phospholipid binding proteins annexin A2 and that C1q also binds A5. These proteins are expressed on apoptotic cells. Further, we have observed that both C1q and factor H bind to the chromatin constituents, DNA and histones. Binding of C1q to these ligands led to complement activation indicating the need for factor H on the apoptotic cells.
NETs constitute one mechanism of how neutrophils can protect the body from pathogens. By releasing chromatin covered with antimicrobial enzymes the neutrophil can catch and kill pathogens. In this thesis we confirm that NETs are not degraded properly in 30% of patients with SLE. Further, C1q binds to NETs, prevents degradation and also activates complement. Consequently, the patients with decreased ability to degrade NETs more often suffer from complement consumption and glomerulonephritis, manifestations of severe SLE. This initial study was followed-up by a longitudinal study where temporal associations such as glomerulonephritis, pleuritis and elevated levels of histone antibodies could then be linked to a decreased ability to degrade NETs.
NET degradation was also affected in some patients with antiphospholipid syndrome and systemic sclerosis. However the effect was not as pronounced as in SLE and was more evident the more SLE-like the APS was.
In conclusion, complement may both prevent and contribute to the pathogenesis in SLE. In this thesis, ligands for complement C1q and factor H have been revealed on the apoptotic cells and further, the interactions of complement with NETs have been elucidated. Additionally, the ability to degrade NETs has been analyzed in SLE and SLE like diseases, which may be used to better diagnose or treat these patients in the future.

Details

Authors
  • Jonatan Leffler
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Other Basic Medicine

Keywords

  • annexins, apoptosis, Complement system, neutrophil extracellular traps, systemic lupus erythematosus, C1q, factor H, histones
Original languageEnglish
QualificationDoctor
Awarding Institution
Supervisors/Assistant supervisor
Award date2013 Apr 26
Publisher
  • Protein Chemistry, Lund University
Print ISBNs978-91-87449-07-9
Publication statusPublished - 2013
Publication categoryResearch

Bibliographic note

Defence details Date: 2013-04-26 Time: 13:00 Place: Lilla Aulan, Jan Walderströmsgata 5, Malmö External reviewer(s) Name: Tedesco, Francesco Title: prof. Affiliation: Department of Physiology and Pathology, University of Trieste, Italy ---

Related research output

Myriam Martin, Leffler, J. & Anna Blom, 2012, In : Journal of Biological Chemistry. 287, 40, p. 33733-33744

Research output: Contribution to journalArticle

Leffler, J., Herbert, A. P., Norström, E., Schmidt, C. Q., Barlow, P. N., Anna Blom & Myriam Martin, 2010, In : Journal of Biological Chemistry. 285, 6, p. 3766-3776

Research output: Contribution to journalArticle

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