Complementary Signaling through flt3 and Interleukin-7 Receptor {alpha} Is Indispensable for Fetal and Adult B Cell Genesis.

Research output: Contribution to journalArticle

Abstract

Extensive studies of mice deficient in one or several cytokine receptors have failed to support an indispensable role of cytokines in development of multiple blood cell lineages. Whereas B1 B cells and Igs are sustained at normal levels throughout life of mice deficient in IL-7, IL-7R{alpha}, common cytokine receptor gamma chain, or flt3 ligand (FL), we report here that adult mice double deficient in IL-7R{alpha} and FL completely lack visible LNs, conventional IgM+ B cells, IgA+ plasma cells, and B1 cells, and consequently produce no Igs. All stages of committed B cell progenitors are undetectable in FL-/- x IL-7R{alpha}-/- BM that also lacks expression of the B cell commitment factor Pax5 and its direct target genes. Furthermore, in contrast to IL-7R{alpha}-/- mice, FL-/- x IL-7R{alpha}-/- mice also lack mature B cells and detectable committed B cell progenitors during fetal development. Thus, signaling through the cytokine tyrosine kinase receptor flt3 and IL-7R{alpha} are indispensable for fetal and adult B cell development.

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Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Immunology in the medical area
  • Cell and Molecular Biology
  • Endocrinology and Diabetes

Keywords

  • IL-7 receptor, Flt3 ligand, B1 cells, Pax5, lymphopoiesis
Original languageEnglish
Pages (from-to)1495-1506
JournalJournal of Experimental Medicine
Volume198
Issue number10
Publication statusPublished - 2003
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Endocrinology (013241500), Hematopoietic Stem Cell Laboratory (013022012), Immunology (013212020), Neurology, Lund (013027000)

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