Compositional differences between infant and adult human corneal basement membranes

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Compositional differences between infant and adult human corneal basement membranes. / Kabosova, Andrea; Azar, Dimitri T.; Bannikov, Gregory A.; Campbell, Kevin P.; Durbeej-Hjalt, Madeleine; Ghohestani, Reza F.; Jones, Jonathan C. R.; Kenney, M. Cristina; Koch, Manuel; Ninomiya, Yoshifumi; Patton, Bruce L.; Paulsson, Mats; Sado, Yoshikazu; Sage, E. Helene; Sasaki, Takako; Sorokin, Lydia M.; Steiner-Champliaud, Marie-France; Sun, Tung-Tien; SundarRaj, Nirmala; Timpl, Rupert; Virtanen, Ismo; Ljubimov, Alexander V.

In: Investigative Ophthalmology & Visual Science, Vol. 48, No. 11, 2007, p. 4989-4999.

Research output: Contribution to journalArticle

Harvard

Kabosova, A, Azar, DT, Bannikov, GA, Campbell, KP, Durbeej-Hjalt, M, Ghohestani, RF, Jones, JCR, Kenney, MC, Koch, M, Ninomiya, Y, Patton, BL, Paulsson, M, Sado, Y, Sage, EH, Sasaki, T, Sorokin, LM, Steiner-Champliaud, M-F, Sun, T-T, SundarRaj, N, Timpl, R, Virtanen, I & Ljubimov, AV 2007, 'Compositional differences between infant and adult human corneal basement membranes', Investigative Ophthalmology & Visual Science, vol. 48, no. 11, pp. 4989-4999. https://doi.org/10.1167/iovs.07-0654

APA

Kabosova, A., Azar, D. T., Bannikov, G. A., Campbell, K. P., Durbeej-Hjalt, M., Ghohestani, R. F., ... Ljubimov, A. V. (2007). Compositional differences between infant and adult human corneal basement membranes. Investigative Ophthalmology & Visual Science, 48(11), 4989-4999. https://doi.org/10.1167/iovs.07-0654

CBE

Kabosova A, Azar DT, Bannikov GA, Campbell KP, Durbeej-Hjalt M, Ghohestani RF, Jones JCR, Kenney MC, Koch M, Ninomiya Y, Patton BL, Paulsson M, Sado Y, Sage EH, Sasaki T, Sorokin LM, Steiner-Champliaud M-F, Sun T-T, SundarRaj N, Timpl R, Virtanen I, Ljubimov AV. 2007. Compositional differences between infant and adult human corneal basement membranes. Investigative Ophthalmology & Visual Science. 48(11):4989-4999. https://doi.org/10.1167/iovs.07-0654

MLA

Vancouver

Author

Kabosova, Andrea ; Azar, Dimitri T. ; Bannikov, Gregory A. ; Campbell, Kevin P. ; Durbeej-Hjalt, Madeleine ; Ghohestani, Reza F. ; Jones, Jonathan C. R. ; Kenney, M. Cristina ; Koch, Manuel ; Ninomiya, Yoshifumi ; Patton, Bruce L. ; Paulsson, Mats ; Sado, Yoshikazu ; Sage, E. Helene ; Sasaki, Takako ; Sorokin, Lydia M. ; Steiner-Champliaud, Marie-France ; Sun, Tung-Tien ; SundarRaj, Nirmala ; Timpl, Rupert ; Virtanen, Ismo ; Ljubimov, Alexander V. / Compositional differences between infant and adult human corneal basement membranes. In: Investigative Ophthalmology & Visual Science. 2007 ; Vol. 48, No. 11. pp. 4989-4999.

RIS

TY - JOUR

T1 - Compositional differences between infant and adult human corneal basement membranes

AU - Kabosova, Andrea

AU - Azar, Dimitri T.

AU - Bannikov, Gregory A.

AU - Campbell, Kevin P.

AU - Durbeej-Hjalt, Madeleine

AU - Ghohestani, Reza F.

AU - Jones, Jonathan C. R.

AU - Kenney, M. Cristina

AU - Koch, Manuel

AU - Ninomiya, Yoshifumi

AU - Patton, Bruce L.

AU - Paulsson, Mats

AU - Sado, Yoshikazu

AU - Sage, E. Helene

AU - Sasaki, Takako

AU - Sorokin, Lydia M.

AU - Steiner-Champliaud, Marie-France

AU - Sun, Tung-Tien

AU - SundarRaj, Nirmala

AU - Timpl, Rupert

AU - Virtanen, Ismo

AU - Ljubimov, Alexander V.

PY - 2007

Y1 - 2007

N2 - PURPOSE. Adult human corneal epithelial basement membrane ( EBM) and Descemet's membrane ( DM) components exhibit heterogeneous distribution. The purpose of the study was to identify changes of these components during postnatal corneal development. METHODS. Thirty healthy adult corneas and 10 corneas from 12-day- to 3-year-old children were studied by immunofluorescence with antibodies against BM components. RESULTS. Type IV collagen composition of infant corneal central EBM over Bowman's layer changed from alpha 1-alpha 2 to alpha 3-alpha 4 chains after 3 years of life; in the adult, alpha 1-alpha 2 chains were retained only in the limbal BM. Laminin alpha 2 and beta 2 chains were present in the adult limbal BM where epithelial stem cells are located. By 3 years of age, beta 2 chain appeared in the limbal BM. In all corneas, limbal BM contained laminin gamma 3 chain. In the infant DM, type IV collagen alpha 1-alpha 6 chains, perlecan, nidogen-1, nidogen-2, and netrin-4 were found on both faces, but they remained only on the endothelial face of the adult DM. The stromal face of the infant but not the adult DM was positive for tenascin-C, fibrillin-1, SPARC, and laminin-332. Type VIII collagen shifted from the endothelial face of infant DM to its stromal face in the adult. Matrilin-4 largely disappeared after the age of 3 years. CONCLUSIONS. The distribution of laminin gamma 3 chain, nidogen-2, netrin-4, matrilin-2, and matrilin-4 is described in the cornea for the first time. The observed differences between adult and infant corneal BMs may relate to changes in their mechanical strength, corneal cell adhesion and differentiation in the process of postnatal corneal maturation.

AB - PURPOSE. Adult human corneal epithelial basement membrane ( EBM) and Descemet's membrane ( DM) components exhibit heterogeneous distribution. The purpose of the study was to identify changes of these components during postnatal corneal development. METHODS. Thirty healthy adult corneas and 10 corneas from 12-day- to 3-year-old children were studied by immunofluorescence with antibodies against BM components. RESULTS. Type IV collagen composition of infant corneal central EBM over Bowman's layer changed from alpha 1-alpha 2 to alpha 3-alpha 4 chains after 3 years of life; in the adult, alpha 1-alpha 2 chains were retained only in the limbal BM. Laminin alpha 2 and beta 2 chains were present in the adult limbal BM where epithelial stem cells are located. By 3 years of age, beta 2 chain appeared in the limbal BM. In all corneas, limbal BM contained laminin gamma 3 chain. In the infant DM, type IV collagen alpha 1-alpha 6 chains, perlecan, nidogen-1, nidogen-2, and netrin-4 were found on both faces, but they remained only on the endothelial face of the adult DM. The stromal face of the infant but not the adult DM was positive for tenascin-C, fibrillin-1, SPARC, and laminin-332. Type VIII collagen shifted from the endothelial face of infant DM to its stromal face in the adult. Matrilin-4 largely disappeared after the age of 3 years. CONCLUSIONS. The distribution of laminin gamma 3 chain, nidogen-2, netrin-4, matrilin-2, and matrilin-4 is described in the cornea for the first time. The observed differences between adult and infant corneal BMs may relate to changes in their mechanical strength, corneal cell adhesion and differentiation in the process of postnatal corneal maturation.

U2 - 10.1167/iovs.07-0654

DO - 10.1167/iovs.07-0654

M3 - Article

VL - 48

SP - 4989

EP - 4999

JO - Investigative Ophthalmology & Visual Science

JF - Investigative Ophthalmology & Visual Science

SN - 1552-5783

IS - 11

ER -