Comprehensive DNA methylation study identifies novel progression-related and prognostic markers for cutaneous melanoma

Research output: Contribution to journalArticle

Abstract

Background: Cutaneous melanoma is the deadliest skin cancer, with an increasing incidence and mortality rate. Currently, staging of patients with primary melanoma is performed using histological biomarkers such as tumor thickness and ulceration. As disruption of the epigenomic landscape is recognized as a widespread feature inherent in tumor development and progression, we aimed to identify novel biomarkers providing additional clinical information over current factors using unbiased genome-wide DNA methylation analyses. Methods: We performed a comprehensive DNA methylation analysis during all progression stages of melanoma using Infinium HumanMethylation450 BeadChips on a discovery cohort of benign nevi (n = 14) and malignant melanoma from both primary (n = 33) and metastatic (n = 28) sites, integrating the DNA methylome with gene expression data. We validated the discovered biomarkers in three independent validation cohorts by pyrosequencing and immunohistochemistry. Results: We identified and validated biomarkers for, and pathways involved in, melanoma development (e.g., HOXA9 DNA methylation) and tumor progression (e.g., TBC1D16 DNA methylation). In addition, we determined a prognostic signature with potential clinical applicability and validated PON3 DNA methylation and OVOL1 protein expression as biomarkers with prognostic information independent of tumor thickness and ulceration. Conclusions: Our data underscores the importance of epigenomic regulation in triggering metastatic dissemination through the inactivation of central cancer-related pathways. Inactivation of cell-adhesion and differentiation unleashes dissemination, and subsequent activation of inflammatory and immune system programs impairs anti-tumoral defense pathways. Moreover, we identify several markers of tumor development and progression previously unrelated to melanoma, and determined a prognostic signature with potential clinical utility.

Details

Authors
  • Jasper Wouters
  • Miguel Vizoso
  • Anna Martinez-Cardus
  • F Javier Carmona
  • Olivier Govaere
  • Teresa Laguna
  • Jesuchristopher Joseph
  • Peter Dynoodt
  • Claudia Aura
  • Mona Foth
  • Roy Cloots
  • Karin van den Hurk
  • Balazs Balint
  • Ian G. Murphy
  • Enda W. McDermott
  • Kieran Sheahan
  • Girish Mallya-Udupi
  • Joost J Van den Oord
  • William M Gallagher
  • Manel Esteller
Organisations
External organisations
  • Catholic University of Leuven
  • OncoMark Ltd
  • Bellvitge Biomedical Research Institute
  • University of Mainz
  • Cancer Research UK
  • Maastricht University
  • St Vincent's University Hospital
  • University College Dublin
  • University of Barcelona
  • Catalan Institution for Research and Advanced Studies
  • Skåne University Hospital
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cancer and Oncology

Keywords

  • Correlation of clinical and molecular markers, Melanoma/skin cancers, Molecular diagnosis and prognosis, Molecular markers of metastasis and progression, Tumor staging
Original languageEnglish
Article number101
Pages (from-to)1-16
JournalBMC Medicine
Volume15
Issue number1
Publication statusPublished - 2017 Jun 5
Publication categoryResearch
Peer-reviewedYes