Confirmation of Protein Biomarkers of Corticosteroids Treatment in Veal Calves Sampled under Field Conditions

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Confirmation of Protein Biomarkers of Corticosteroids Treatment in Veal Calves Sampled under Field Conditions. / Stella, Roberto; Arrigoni, Giorgio; Biancotto, Giancarlo; Krogh, Morten; Vascellari, Marta; Lega, Francesca; Pozza, Giandomenico; Angeletti, Roberto; Andrighetto, Igino; James, Peter.

In: Journal of Proteome Research, Vol. 13, No. 4, 2014, p. 1794-1799.

Research output: Contribution to journalArticle

Harvard

Stella, R, Arrigoni, G, Biancotto, G, Krogh, M, Vascellari, M, Lega, F, Pozza, G, Angeletti, R, Andrighetto, I & James, P 2014, 'Confirmation of Protein Biomarkers of Corticosteroids Treatment in Veal Calves Sampled under Field Conditions', Journal of Proteome Research, vol. 13, no. 4, pp. 1794-1799. https://doi.org/10.1021/pr401193r

APA

CBE

Stella R, Arrigoni G, Biancotto G, Krogh M, Vascellari M, Lega F, Pozza G, Angeletti R, Andrighetto I, James P. 2014. Confirmation of Protein Biomarkers of Corticosteroids Treatment in Veal Calves Sampled under Field Conditions. Journal of Proteome Research. 13(4):1794-1799. https://doi.org/10.1021/pr401193r

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Author

Stella, Roberto ; Arrigoni, Giorgio ; Biancotto, Giancarlo ; Krogh, Morten ; Vascellari, Marta ; Lega, Francesca ; Pozza, Giandomenico ; Angeletti, Roberto ; Andrighetto, Igino ; James, Peter. / Confirmation of Protein Biomarkers of Corticosteroids Treatment in Veal Calves Sampled under Field Conditions. In: Journal of Proteome Research. 2014 ; Vol. 13, No. 4. pp. 1794-1799.

RIS

TY - JOUR

T1 - Confirmation of Protein Biomarkers of Corticosteroids Treatment in Veal Calves Sampled under Field Conditions

AU - Stella, Roberto

AU - Arrigoni, Giorgio

AU - Biancotto, Giancarlo

AU - Krogh, Morten

AU - Vascellari, Marta

AU - Lega, Francesca

AU - Pozza, Giandomenico

AU - Angeletti, Roberto

AU - Andrighetto, Igino

AU - James, Peter

PY - 2014

Y1 - 2014

N2 - In veal calf production, growth promoters are still illicitly used. Surveillance of misuse of such molecules is necessary to preserve human health. Methods currently adopted for their analysis are based on liquid chromatography tandem mass spectrometry, but their efficacy can be affected by undetectable residual concentrations in biological matrices due to treatments at low-dosage or based on unknown anabolic compounds. The development of screening methods to identify the indirect biological effects of administration of growth promoters can improve the efficiency of drug residue monitoring. To this purpose, an integrated approach has been used to further validate the set of protein biomarkers defined in a previous controlled study to detect the use of corticosteroids through the changes caused in muscle protein expression. The thymus morphology of 48 samples collected under field conditions was evaluated to assess the presence of potential corticosteroids treatment. Animals were divided on the basis of their thymus characteristics in negative or suspected for illegal corticosteroids treatment. Drug residue analyses were performed on the liver, giving a satisfactory correlation with the histological examination (similar to 85%). Finally, the proteomics analysis of muscle protein extracts was carried out by 2D differential in gel electrophoresis, and proteins that were differentially expressed between the two animal groups (p value <0.01) were selected for MALDI-MS/MS analysis. This approach allowed us to identify 29 different proteins, and our findings indicate that the altered protein expression pattern can be used as an indirect method for the detection of illicit corticosteroids administration. A subset of the identified proteins was already reported in a previous controlled study, proving that these biomarkers can be used to develop a screening assay to improve the tools currently available for the detection of corticosteroids abuse in bovine meat production.

AB - In veal calf production, growth promoters are still illicitly used. Surveillance of misuse of such molecules is necessary to preserve human health. Methods currently adopted for their analysis are based on liquid chromatography tandem mass spectrometry, but their efficacy can be affected by undetectable residual concentrations in biological matrices due to treatments at low-dosage or based on unknown anabolic compounds. The development of screening methods to identify the indirect biological effects of administration of growth promoters can improve the efficiency of drug residue monitoring. To this purpose, an integrated approach has been used to further validate the set of protein biomarkers defined in a previous controlled study to detect the use of corticosteroids through the changes caused in muscle protein expression. The thymus morphology of 48 samples collected under field conditions was evaluated to assess the presence of potential corticosteroids treatment. Animals were divided on the basis of their thymus characteristics in negative or suspected for illegal corticosteroids treatment. Drug residue analyses were performed on the liver, giving a satisfactory correlation with the histological examination (similar to 85%). Finally, the proteomics analysis of muscle protein extracts was carried out by 2D differential in gel electrophoresis, and proteins that were differentially expressed between the two animal groups (p value <0.01) were selected for MALDI-MS/MS analysis. This approach allowed us to identify 29 different proteins, and our findings indicate that the altered protein expression pattern can be used as an indirect method for the detection of illicit corticosteroids administration. A subset of the identified proteins was already reported in a previous controlled study, proving that these biomarkers can be used to develop a screening assay to improve the tools currently available for the detection of corticosteroids abuse in bovine meat production.

KW - corticosteroids

KW - veal calves

KW - indirect markers

KW - growth promoters

KW - proteomics

U2 - 10.1021/pr401193r

DO - 10.1021/pr401193r

M3 - Article

VL - 13

SP - 1794

EP - 1799

JO - Journal of Proteome Research

T2 - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 4

ER -