Construction and Characterization of Kilobasepair Densely Labeled Peptide-DNA

Research output: Contribution to journalArticle

Abstract

Directed assembly of biocompatible materials benefits from modular building blocks in which structural organization is independent of introduced functional modifications. For soft materials, such modifications have been limited. Here, long DNA is successfully functionalized with dense decoration by peptides. Following introduction of alkyne-modified nucleotides into kilobasepair DNA, measurements of persistence length show that DNA mechanics are unaltered by the dense incorporation of alkynes (similar to 1 alkyne/2 bp) and after click-chemistry attachment of a tunable density of peptides. Proteolytic cleavage of densely tethered peptides (similar to 1 peptide/3 bp) demonstrates addressability of the functional groups, showing that this accessible approach to creating hybrid structures can maintain orthogonality between backbone mechanics and overlaid function. The synthesis and characterization of these hybrid constructs establishes the groundwork for their implementation in future applications, such as building blocks in modular approaches to a range of problems in synthetic biology.

Details

Authors
  • Suzana Kovacic
  • Laleh Samii
  • Guillaume Lamour
  • Hongbin Li
  • Heiner Linke
  • Elizabeth H. C. Bromley
  • Derek N. Woolfson
  • Paul M. G. Curmi
  • Nancy R. Forde
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Condensed Matter Physics
Original languageEnglish
Pages (from-to)4065-4072
JournalBiomacromolecules
Volume15
Issue number11
Publication statusPublished - 2014
Publication categoryResearch
Peer-reviewedYes