Construction and Characterization of Kilobasepair Densely Labeled Peptide-DNA

Research output: Contribution to journalArticle

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Construction and Characterization of Kilobasepair Densely Labeled Peptide-DNA. / Kovacic, Suzana; Samii, Laleh; Lamour, Guillaume; Li, Hongbin; Linke, Heiner; Bromley, Elizabeth H. C.; Woolfson, Derek N.; Curmi, Paul M. G.; Forde, Nancy R.

In: Biomacromolecules, Vol. 15, No. 11, 2014, p. 4065-4072.

Research output: Contribution to journalArticle

Harvard

Kovacic, S, Samii, L, Lamour, G, Li, H, Linke, H, Bromley, EHC, Woolfson, DN, Curmi, PMG & Forde, NR 2014, 'Construction and Characterization of Kilobasepair Densely Labeled Peptide-DNA', Biomacromolecules, vol. 15, no. 11, pp. 4065-4072. https://doi.org/10.1021/bm501109p

APA

Kovacic, S., Samii, L., Lamour, G., Li, H., Linke, H., Bromley, E. H. C., ... Forde, N. R. (2014). Construction and Characterization of Kilobasepair Densely Labeled Peptide-DNA. Biomacromolecules, 15(11), 4065-4072. https://doi.org/10.1021/bm501109p

CBE

Kovacic S, Samii L, Lamour G, Li H, Linke H, Bromley EHC, Woolfson DN, Curmi PMG, Forde NR. 2014. Construction and Characterization of Kilobasepair Densely Labeled Peptide-DNA. Biomacromolecules. 15(11):4065-4072. https://doi.org/10.1021/bm501109p

MLA

Vancouver

Author

Kovacic, Suzana ; Samii, Laleh ; Lamour, Guillaume ; Li, Hongbin ; Linke, Heiner ; Bromley, Elizabeth H. C. ; Woolfson, Derek N. ; Curmi, Paul M. G. ; Forde, Nancy R. / Construction and Characterization of Kilobasepair Densely Labeled Peptide-DNA. In: Biomacromolecules. 2014 ; Vol. 15, No. 11. pp. 4065-4072.

RIS

TY - JOUR

T1 - Construction and Characterization of Kilobasepair Densely Labeled Peptide-DNA

AU - Kovacic, Suzana

AU - Samii, Laleh

AU - Lamour, Guillaume

AU - Li, Hongbin

AU - Linke, Heiner

AU - Bromley, Elizabeth H. C.

AU - Woolfson, Derek N.

AU - Curmi, Paul M. G.

AU - Forde, Nancy R.

PY - 2014

Y1 - 2014

N2 - Directed assembly of biocompatible materials benefits from modular building blocks in which structural organization is independent of introduced functional modifications. For soft materials, such modifications have been limited. Here, long DNA is successfully functionalized with dense decoration by peptides. Following introduction of alkyne-modified nucleotides into kilobasepair DNA, measurements of persistence length show that DNA mechanics are unaltered by the dense incorporation of alkynes (similar to 1 alkyne/2 bp) and after click-chemistry attachment of a tunable density of peptides. Proteolytic cleavage of densely tethered peptides (similar to 1 peptide/3 bp) demonstrates addressability of the functional groups, showing that this accessible approach to creating hybrid structures can maintain orthogonality between backbone mechanics and overlaid function. The synthesis and characterization of these hybrid constructs establishes the groundwork for their implementation in future applications, such as building blocks in modular approaches to a range of problems in synthetic biology.

AB - Directed assembly of biocompatible materials benefits from modular building blocks in which structural organization is independent of introduced functional modifications. For soft materials, such modifications have been limited. Here, long DNA is successfully functionalized with dense decoration by peptides. Following introduction of alkyne-modified nucleotides into kilobasepair DNA, measurements of persistence length show that DNA mechanics are unaltered by the dense incorporation of alkynes (similar to 1 alkyne/2 bp) and after click-chemistry attachment of a tunable density of peptides. Proteolytic cleavage of densely tethered peptides (similar to 1 peptide/3 bp) demonstrates addressability of the functional groups, showing that this accessible approach to creating hybrid structures can maintain orthogonality between backbone mechanics and overlaid function. The synthesis and characterization of these hybrid constructs establishes the groundwork for their implementation in future applications, such as building blocks in modular approaches to a range of problems in synthetic biology.

U2 - 10.1021/bm501109p

DO - 10.1021/bm501109p

M3 - Article

VL - 15

SP - 4065

EP - 4072

JO - Biomacromolecules

T2 - Biomacromolecules

JF - Biomacromolecules

SN - 1526-4602

IS - 11

ER -