Contractile effects of neuropeptide Y in human subcutaneous resistance arteries are mediated by Y1 receptors

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@article{4066ea9218f84cbd8a480706fecfd862,
title = "Contractile effects of neuropeptide Y in human subcutaneous resistance arteries are mediated by Y1 receptors",
abstract = "The aim of our study was to determine the neuropeptide Y (NPY) receptor subtype responsible for the NPY-induced contraction of human subcutaneous (s.c.) resistance arteries. To elucidate this, we used (a) in vitro studies of NPY agonists: NPY, peptide YY (PYY), and Pro34NPY induced equally strong and equipotent concentration-dependent contractions of human s.c. resistance arteries, whereas NPY13-36 and NPY18-36 had no contractile effects; (b) in vitro studies using the NPY Y1-receptor antagonist, BIBP3226, which in nanomolar concentrations inhibited the contractile effect of NPY, causing a rightward shift of the concentration-response curve. pEC50 for NPY alone, 8.41 +/- 0.21; NPY + BIBP3226, 10 nM, 7.79 +/- 0.21; NPY + BIBP3226, 100 nM, 7.18 +/- 0.18; NPY + BIBP3226, 1 microM, 6.32 +/- 0.05 (n = 5-8). Schild-plot analysis indicated competitive antagonism: pA2 = 8.53 +/- 0.22 and slope = 0.99 +/- 0.14; (c) with reverse transcriptase-polymerase chain reaction (RT-PCR), we detected messenger RNA (mRNA) encoding the human NPY Y1 receptor and a splice variant of the receptor in human s.c. resistance arteries. On the basis of the agonists' potency order, the antagonistic effect of BIBP3226 on the NPY-induced contraction, and the presence of mRNA encoding the NPY Y1 receptor, we conclude that the NPY-induced contraction of human s.c. resistance arteries is mediated by NPY Y1 receptors.",
keywords = "Resistance arteries, Neuropeptide Y, Human, BIBP3226, Reverse transcriptase, Polymerase chain reaction",
author = "Torun Nilsson and David Erlinge and Leonor Cantera and Lars Edvinsson",
year = "1996",
language = "English",
volume = "28",
pages = "764--768",
journal = "Journal of Cardiovascular Pharmacology",
issn = "1533-4023",
publisher = "Lippincott Williams & Wilkins",
number = "6",

}