Contribution of Antibody-based Protein Profiling to the Human Chromosome-centric Proteome Project (C-HPP)

Research output: Contribution to journalArticle

Abstract

A gene-centric Human Proteome Project has been proposed to characterize the human protein-coding genes in a chromosome-centered manner to understand human biology and disease. Here, we report on the protein evidence for all genes predicted from the genome sequence based on manual annotation from literature (UniProt), antibody-based profiling in cells, tissues and organs and analysis of the transcript profiles using next generation sequencing in human cell lines of different origins. We estimate that there is good evidence for protein existence for 69% (n = 13985) of the human protein-coding genes, while 23% have only evidence on the RNA level and 7% still lack experimental evidence. Analysis of the expression patterns shows few regards to protein evidence is visualized in a chromosome-centric manner as part of a new version of the Human Protein Atlas (www.proteinatlas.org).

Details

Authors
  • Linn Fagerberg
  • Per Oksvold
  • Marie Skogs
  • Cajsa Algenas
  • Emma Lundberg
  • Fredrik Ponten
  • Asa Sivertsson
  • Jacob Odeberg
  • Daniel Klevebring
  • Caroline Kampf
  • Anna Asplund
  • Evelina Sjostedt
  • Cristina Al-Khalili Szigyarto
  • Per-Henrik Edqvist
  • IngMarie Olsson
  • Urban Rydberg
  • Paul Hudson
  • Jenny Ottosson Takanen
  • Holger Berling
  • Lisa Bjorling
  • Hanna Tegel
  • Johan Rockberg
  • Peter Nilsson
  • Sanjay Navani
  • Jan Mulder
  • Jochen M. Schwenk
  • Martin Zwahlen
  • Sophia Hober
  • Mattias Forsberg
  • Kalle von Feilitzen
  • Mathias Uhlen
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cancer and Oncology

Keywords

  • Antibody-based protein profiling, C-HPP
Original languageEnglish
Pages (from-to)2439-2448
JournalJournal of Proteome Research
Volume12
Issue number6
Publication statusPublished - 2013
Publication categoryResearch
Peer-reviewedYes

Bibliographic note

The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000)