Cost Analysis of Ethanol Production from Willow Using Recombinant Escherichia coli

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Cost Analysis of Ethanol Production from Willow Using Recombinant Escherichia coli. / von Sivers, M; Zacchi, Guido; Olsson, L; Hahn-Hägerdal, Bärbel.

In: Biotechnology Progress, Vol. 10, No. 5, 1994, p. 555-560.

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TY - JOUR

T1 - Cost Analysis of Ethanol Production from Willow Using Recombinant Escherichia coli

AU - von Sivers, M

AU - Zacchi, Guido

AU - Olsson, L

AU - Hahn-Hägerdal, Bärbel

PY - 1994

Y1 - 1994

N2 - This study comprises a technical and economic analysis of the production of fuel ethanol by fermentation of a pentose-rich hydrolysate with recombinant Escherichia coli, strain KO11. Hydrolysate from steam-pretreated willow was used as raw material in calculations regarding the fermentation. The calculations were based on a feed capacity of 10 metric tons of dry willow per hour to the pretreatment stage, providing 35 metric tons of hydrolysate per hour, consisting of 45 g of sugars/L, to the pentose fermentation plant. A detoxification step was included, since the hydrolysate has been shown to have an inhibitory effect on the E. coli KO11. The technical data used in the calculations were based on a kinetic fermentation model, which was developed from laboratory-scale experiments in a previous study. The economic analysis predicted an ethanol production cast of 48 cent/L in the pentose fermentation plant, indicating potentially good economy. The detoxification cost constitutes 22% of this cost. Sensitivity analyses revealed that if the concentration of sugars in the feed to the fermentation was decreased by 40% to 27 g/L, the ethanol production cost was increased to 54 cent/L. The production cost was increased to 50 cent/L ethanol if the cell mass was recirculated to the fermentation stage 5 times instead of 20.

AB - This study comprises a technical and economic analysis of the production of fuel ethanol by fermentation of a pentose-rich hydrolysate with recombinant Escherichia coli, strain KO11. Hydrolysate from steam-pretreated willow was used as raw material in calculations regarding the fermentation. The calculations were based on a feed capacity of 10 metric tons of dry willow per hour to the pretreatment stage, providing 35 metric tons of hydrolysate per hour, consisting of 45 g of sugars/L, to the pentose fermentation plant. A detoxification step was included, since the hydrolysate has been shown to have an inhibitory effect on the E. coli KO11. The technical data used in the calculations were based on a kinetic fermentation model, which was developed from laboratory-scale experiments in a previous study. The economic analysis predicted an ethanol production cast of 48 cent/L in the pentose fermentation plant, indicating potentially good economy. The detoxification cost constitutes 22% of this cost. Sensitivity analyses revealed that if the concentration of sugars in the feed to the fermentation was decreased by 40% to 27 g/L, the ethanol production cost was increased to 54 cent/L. The production cost was increased to 50 cent/L ethanol if the cell mass was recirculated to the fermentation stage 5 times instead of 20.

U2 - 10.1021/bp00029a017

DO - 10.1021/bp00029a017

M3 - Article

VL - 10

SP - 555

EP - 560

JO - Biotechnology Progress

JF - Biotechnology Progress

SN - 1520-6033

IS - 5

ER -