Critical function for Naip5 in inflammasome activation by a conserved carboxy-terminal domain of flagellin

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Critical function for Naip5 in inflammasome activation by a conserved carboxy-terminal domain of flagellin. / Lightfield, Karla L; Persson, Jenny; Brubaker, Sky W; Witte, Chelsea E; von Moltke, Jakob; Dunipace, Eric A; Henry, Thomas; Sun, Yao-Hui; Cado, Dragana; Dietrich, William F; Monack, Denise M; Tsolis, Renée M; Vance, Russell E.

In: Nature Immunology, Vol. 9, No. 10, 10.2008, p. 1171-8.

Research output: Contribution to journalArticle

Harvard

Lightfield, KL, Persson, J, Brubaker, SW, Witte, CE, von Moltke, J, Dunipace, EA, Henry, T, Sun, Y-H, Cado, D, Dietrich, WF, Monack, DM, Tsolis, RM & Vance, RE 2008, 'Critical function for Naip5 in inflammasome activation by a conserved carboxy-terminal domain of flagellin', Nature Immunology, vol. 9, no. 10, pp. 1171-8. https://doi.org/10.1038/ni.1646

APA

Lightfield, K. L., Persson, J., Brubaker, S. W., Witte, C. E., von Moltke, J., Dunipace, E. A., Henry, T., Sun, Y-H., Cado, D., Dietrich, W. F., Monack, D. M., Tsolis, R. M., & Vance, R. E. (2008). Critical function for Naip5 in inflammasome activation by a conserved carboxy-terminal domain of flagellin. Nature Immunology, 9(10), 1171-8. https://doi.org/10.1038/ni.1646

CBE

Lightfield KL, Persson J, Brubaker SW, Witte CE, von Moltke J, Dunipace EA, Henry T, Sun Y-H, Cado D, Dietrich WF, Monack DM, Tsolis RM, Vance RE. 2008. Critical function for Naip5 in inflammasome activation by a conserved carboxy-terminal domain of flagellin. Nature Immunology. 9(10):1171-8. https://doi.org/10.1038/ni.1646

MLA

Vancouver

Author

Lightfield, Karla L ; Persson, Jenny ; Brubaker, Sky W ; Witte, Chelsea E ; von Moltke, Jakob ; Dunipace, Eric A ; Henry, Thomas ; Sun, Yao-Hui ; Cado, Dragana ; Dietrich, William F ; Monack, Denise M ; Tsolis, Renée M ; Vance, Russell E. / Critical function for Naip5 in inflammasome activation by a conserved carboxy-terminal domain of flagellin. In: Nature Immunology. 2008 ; Vol. 9, No. 10. pp. 1171-8.

RIS

TY - JOUR

T1 - Critical function for Naip5 in inflammasome activation by a conserved carboxy-terminal domain of flagellin

AU - Lightfield, Karla L

AU - Persson, Jenny

AU - Brubaker, Sky W

AU - Witte, Chelsea E

AU - von Moltke, Jakob

AU - Dunipace, Eric A

AU - Henry, Thomas

AU - Sun, Yao-Hui

AU - Cado, Dragana

AU - Dietrich, William F

AU - Monack, Denise M

AU - Tsolis, Renée M

AU - Vance, Russell E

PY - 2008/10

Y1 - 2008/10

N2 - Inflammasomes are cytosolic multiprotein complexes that sense microbial infection and trigger cytokine production and cell death. However, the molecular components of inflammasomes and what they sense remain poorly defined. Here we demonstrate that 35 amino acids of the carboxyl terminus of flagellin triggered inflammasome activation in the absence of bacterial contaminants or secretion systems. To further elucidate the host flagellin-sensing pathway, we generated mice deficient in the intracellular sensor Naip5. These mice failed to activate the inflammasome in response to the 35 amino acids of flagellin or in response to Legionella pneumophila infection. Our data clarify the molecular basis for the cytosolic response to flagellin.

AB - Inflammasomes are cytosolic multiprotein complexes that sense microbial infection and trigger cytokine production and cell death. However, the molecular components of inflammasomes and what they sense remain poorly defined. Here we demonstrate that 35 amino acids of the carboxyl terminus of flagellin triggered inflammasome activation in the absence of bacterial contaminants or secretion systems. To further elucidate the host flagellin-sensing pathway, we generated mice deficient in the intracellular sensor Naip5. These mice failed to activate the inflammasome in response to the 35 amino acids of flagellin or in response to Legionella pneumophila infection. Our data clarify the molecular basis for the cytosolic response to flagellin.

KW - Amino Acid Motifs/immunology

KW - Animals

KW - Apoptosis Regulatory Proteins/immunology

KW - Calcium-Binding Proteins/immunology

KW - Cytosol

KW - Enzyme-Linked Immunosorbent Assay

KW - Flagellin/chemistry

KW - Immunoblotting

KW - Legionella pneumophila/immunology

KW - Legionnaires' Disease/immunology

KW - Macrophages/immunology

KW - Mice

KW - Multiprotein Complexes/immunology

KW - Neuronal Apoptosis-Inhibitory Protein/genetics

KW - Toll-Like Receptor 5/immunology

KW - Transduction, Genetic

U2 - 10.1038/ni.1646

DO - 10.1038/ni.1646

M3 - Article

C2 - 18724372

VL - 9

SP - 1171

EP - 1178

JO - Nature Immunology

JF - Nature Immunology

SN - 1529-2908

IS - 10

ER -