Crystallization and preliminary X-ray analysis of human dUTPase

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Human dUTPase, expressed in Escherichia coli, has been crystallized. Single crystals were obtained by the vapour-diffusion technique using 2-propanol and PEG 4000 as precipitants. The enzyme was co-crystallized with the substrate dUTP and a metal chelator EDTA to prevent hydrolysis of the substrate. The crystals belong to the orthorombic space group P212121 with cell dimensions a = 67.51, b = 68.26 and c = 91.00 Å. The crystallographic asymmetric unit contains one trimer of identical subunits.


  • G Larsson
  • Per-Olof Nyman
  • L A Svensson
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Structural Biology
Original languageEnglish
Pages (from-to)1039-1040
JournalActa Crystallographica. Section D: Biological Crystallography
Issue number5
Publication statusPublished - 1996
Publication categoryResearch