Crystallization and preliminary X-ray analysis of human dUTPase
Research output: Contribution to journal › Article
Human dUTPase, expressed in Escherichia coli, has been crystallized. Single crystals were obtained by the vapour-diffusion technique using 2-propanol and PEG 4000 as precipitants. The enzyme was co-crystallized with the substrate dUTP and a metal chelator EDTA to prevent hydrolysis of the substrate. The crystals belong to the orthorombic space group P212121 with cell dimensions a = 67.51, b = 68.26 and c = 91.00 Å. The crystallographic asymmetric unit contains one trimer of identical subunits.
|Research areas and keywords||
Subject classification (UKÄ) – MANDATORY
|Journal||Acta Crystallographica. Section D: Biological Crystallography|
|Publication status||Published - 1996|