Crystallization and preliminary X-ray analysis of human dUTPase

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Crystallization and preliminary X-ray analysis of human dUTPase. / Larsson, G; Nyman, Per-Olof; Svensson, L A.

In: Acta Crystallographica. Section D: Biological Crystallography, Vol. D52, No. 5, 1996, p. 1039-1040.

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Larsson, G ; Nyman, Per-Olof ; Svensson, L A. / Crystallization and preliminary X-ray analysis of human dUTPase. In: Acta Crystallographica. Section D: Biological Crystallography. 1996 ; Vol. D52, No. 5. pp. 1039-1040.

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TY - JOUR

T1 - Crystallization and preliminary X-ray analysis of human dUTPase

AU - Larsson, G

AU - Nyman, Per-Olof

AU - Svensson, L A

PY - 1996

Y1 - 1996

N2 - Human dUTPase, expressed in Escherichia coli, has been crystallized. Single crystals were obtained by the vapour-diffusion technique using 2-propanol and PEG 4000 as precipitants. The enzyme was co-crystallized with the substrate dUTP and a metal chelator EDTA to prevent hydrolysis of the substrate. The crystals belong to the orthorombic space group P212121 with cell dimensions a = 67.51, b = 68.26 and c = 91.00 Å. The crystallographic asymmetric unit contains one trimer of identical subunits.

AB - Human dUTPase, expressed in Escherichia coli, has been crystallized. Single crystals were obtained by the vapour-diffusion technique using 2-propanol and PEG 4000 as precipitants. The enzyme was co-crystallized with the substrate dUTP and a metal chelator EDTA to prevent hydrolysis of the substrate. The crystals belong to the orthorombic space group P212121 with cell dimensions a = 67.51, b = 68.26 and c = 91.00 Å. The crystallographic asymmetric unit contains one trimer of identical subunits.

U2 - 10.1107/S0907444996003277

DO - 10.1107/S0907444996003277

M3 - Article

VL - D52

SP - 1039

EP - 1040

JO - Acta Crystallographica Section D: Structural Biology

JF - Acta Crystallographica Section D: Structural Biology

SN - 2059-7983

IS - 5

ER -