Crystallization and Structure Determination of Superantigens and Immune Receptor Complexes.

Research output: Contribution to journalArticle

Abstract

Structure determination of superantigens and the complexes they form with immune receptors have over the years provided insight in their modes of action. This technique requires growing large and highly ordered crystals of the superantigen or receptor-superantigen complex, followed by exposure to X-ray radiation and data collection. Here, we describe methods for crystallizing superantigens and superantigen-receptor complexes using the vapor diffusion technique, how the crystals may be optimized, and lastly data collection and structure determination.

Details

Authors
Organisations
External organisations
  • University of Copenhagen
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell and Molecular Biology
Original languageEnglish
Pages (from-to)51-65
JournalMethods in Molecular Biology
Volume1396
Publication statusPublished - 2016
Publication categoryResearch
Peer-reviewedYes