Crystallization of a stringent response factor from Aquifex aeolicus.

Research output: Contribution to journalArticle

Abstract

The crystallization of a key enzyme from Aquifex aeolicus with suggested bifunctional activity, acting as an exopolyphosphatase and a guanosine pentaphosphate phosphohydrolase, is reported. Native data were collected to below 2 A resolution from an orthorhombic crystal with unit-cell parameters a = 50.8, b = 70.3, c = 90.9 A. Methionine residues were introduced by mutation and deliberate oxidation of the protein allowed us to produce additional crystal forms with reproducible diffraction ability and increased phasing potential. This is the first report on the crystallization of a member of the Ppx/GppA phosphatase family.

Details

Authors
  • Ole Kristensen
  • Martin Laurberg
  • Michael Gajhede
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Structural Biology
Original languageEnglish
Pages (from-to)1198-1200
JournalActa Crystallographica. Section D: Biological Crystallography
Volume58
Issue numberPt 7
Publication statusPublished - 2002
Publication categoryResearch
Peer-reviewedYes