Cubosomes for in vivo fluorescence lifetime imaging

Research output: Contribution to journalArticle

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Cubosomes for in vivo fluorescence lifetime imaging. / Biffi, Stefania; Andolfi, Laura; Caltagirone, Claudia; Garrovo, Chiara; Falchi, Angela M; Lippolis, Vito; Lorenzon, Andrea; Macor, Paolo; Meli, Valeria; Monduzzi, Maura; Obiols-Rabasa, Marc; Petrizza, Luca; Prodi, Luca; Rosa, Antonella; Schmidt, Judith; Talmon, Yeshayahu; Murgia, Sergio.

In: Nanotechnology, Vol. 28, No. 5, 055102, 03.02.2017.

Research output: Contribution to journalArticle

Harvard

Biffi, S, Andolfi, L, Caltagirone, C, Garrovo, C, Falchi, AM, Lippolis, V, Lorenzon, A, Macor, P, Meli, V, Monduzzi, M, Obiols-Rabasa, M, Petrizza, L, Prodi, L, Rosa, A, Schmidt, J, Talmon, Y & Murgia, S 2017, 'Cubosomes for in vivo fluorescence lifetime imaging', Nanotechnology, vol. 28, no. 5, 055102. https://doi.org/10.1088/1361-6528/28/5/055102

APA

Biffi, S., Andolfi, L., Caltagirone, C., Garrovo, C., Falchi, A. M., Lippolis, V., ... Murgia, S. (2017). Cubosomes for in vivo fluorescence lifetime imaging. Nanotechnology, 28(5), [055102]. https://doi.org/10.1088/1361-6528/28/5/055102

CBE

Biffi S, Andolfi L, Caltagirone C, Garrovo C, Falchi AM, Lippolis V, Lorenzon A, Macor P, Meli V, Monduzzi M, Obiols-Rabasa M, Petrizza L, Prodi L, Rosa A, Schmidt J, Talmon Y, Murgia S. 2017. Cubosomes for in vivo fluorescence lifetime imaging. Nanotechnology. 28(5). https://doi.org/10.1088/1361-6528/28/5/055102

MLA

Vancouver

Biffi S, Andolfi L, Caltagirone C, Garrovo C, Falchi AM, Lippolis V et al. Cubosomes for in vivo fluorescence lifetime imaging. Nanotechnology. 2017 Feb 3;28(5). 055102. https://doi.org/10.1088/1361-6528/28/5/055102

Author

Biffi, Stefania ; Andolfi, Laura ; Caltagirone, Claudia ; Garrovo, Chiara ; Falchi, Angela M ; Lippolis, Vito ; Lorenzon, Andrea ; Macor, Paolo ; Meli, Valeria ; Monduzzi, Maura ; Obiols-Rabasa, Marc ; Petrizza, Luca ; Prodi, Luca ; Rosa, Antonella ; Schmidt, Judith ; Talmon, Yeshayahu ; Murgia, Sergio. / Cubosomes for in vivo fluorescence lifetime imaging. In: Nanotechnology. 2017 ; Vol. 28, No. 5.

RIS

TY - JOUR

T1 - Cubosomes for in vivo fluorescence lifetime imaging

AU - Biffi, Stefania

AU - Andolfi, Laura

AU - Caltagirone, Claudia

AU - Garrovo, Chiara

AU - Falchi, Angela M

AU - Lippolis, Vito

AU - Lorenzon, Andrea

AU - Macor, Paolo

AU - Meli, Valeria

AU - Monduzzi, Maura

AU - Obiols-Rabasa, Marc

AU - Petrizza, Luca

AU - Prodi, Luca

AU - Rosa, Antonella

AU - Schmidt, Judith

AU - Talmon, Yeshayahu

AU - Murgia, Sergio

PY - 2017/2/3

Y1 - 2017/2/3

N2 - Herein we provided the first proof of principle for in vivo fluorescence optical imaging application using monoolein-based cubosomes in a healthy mouse animal model. This formulation, administered at a non-cytotoxic concentration, was capable of providing both exogenous contrast for NIR fluorescence imaging with very high efficiency and chemospecific information upon lifetime analysis. Time-resolved measurements of fluorescence after the intravenous injection of cubosomes revealed that the dye rapidly accumulated mainly in the liver, while lifetimes profiles obtained in vivo allowed for discriminating between free dye or dye embedded within the cubosome nanostructure after injection.

AB - Herein we provided the first proof of principle for in vivo fluorescence optical imaging application using monoolein-based cubosomes in a healthy mouse animal model. This formulation, administered at a non-cytotoxic concentration, was capable of providing both exogenous contrast for NIR fluorescence imaging with very high efficiency and chemospecific information upon lifetime analysis. Time-resolved measurements of fluorescence after the intravenous injection of cubosomes revealed that the dye rapidly accumulated mainly in the liver, while lifetimes profiles obtained in vivo allowed for discriminating between free dye or dye embedded within the cubosome nanostructure after injection.

KW - fluorescence lifetime probe

KW - fluorescence optical imaging in vivo

KW - liquid crystalline nanoparticles

UR - http://www.scopus.com/inward/record.url?scp=85009113821&partnerID=8YFLogxK

U2 - 10.1088/1361-6528/28/5/055102

DO - 10.1088/1361-6528/28/5/055102

M3 - Article

VL - 28

JO - Nanotechnology

T2 - Nanotechnology

JF - Nanotechnology

SN - 0957-4484

IS - 5

M1 - 055102

ER -