Culture in embryonic kidney serum and xeno-free media as renal cell carcinoma and renal cell carcinoma cancer stem cells research model

Research output: Contribution to journalArticle

Abstract

The use of fetal bovine serum hinders obtaining reproducible experimental results and should also be removed in hormone and growth factor studies. In particular hormones found in FBS act globally on cancer cell physiology and influence transcriptome and metabolome. The aim of our study was to develop a renal carcinoma serum free culture model optimized for (embryonal) renal cells in order to select the best study model for downstream auto-, para- or endocrine research. Secondary aim was to verify renal carcinoma stem cell culture for this application. In the study, we have cultured renal cell carcinoma primary tumour cell line (786-0) as well as human kidney cancer stem cells in standard 2D monolayer cultures in Roswell Park Memorial Institute Medium or Dulbecco’s Modified Eagle’s Medium and Complete Human Kidney Cancer Stem Cell Medium, respectively. Serum-free, animal-component free Human Embryonic Kidney 293 media were tested. Our results revealed that xeno-free embryonal renal cells optimized culture media provide a useful tool in RCC cancer biology research and at the same time enable effective growth of RCC. We propose bio-mimic RCC cell culture model with specific serum-free and xeno-free medium that promote RCC cell viability.

Details

Authors
  • Krzysztof M. Krawczyk
  • Damian Matak
  • Lukasz Szymanski
  • Cezary Szczylik
  • Camillo Porta
  • Anna M. Czarnecka
Organisations
External organisations
  • Medical University of Warsaw
  • Policlinico San Matteo Pavia Fondazione
  • Military Institute of Medicine
  • University of Warsaw
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cancer and Oncology

Keywords

  • Cancer stem cells, Renal cell cancer, Serum-free culture, Xeno-free culture
Original languageEnglish
Pages (from-to)761-782
JournalCytotechnology
Volume70
Issue number2
Early online date2018 Feb 17
Publication statusPublished - 2018
Publication categoryResearch
Peer-reviewedYes