Culturing and patch clamping of Jurkat T cells and neurons on Al 2 O 3 coated nanowire arrays of altered morphology

Research output: Contribution to journalArticle

Abstract

Nanowire substrates play an increasingly important role for cell cultures as an approach for hybrid bio-semiconductor junctions. We investigate Jurkat T cells and neurons from mice cultured on Al 2 O 3 coated ordered and randomly distributed nanowires. Cell viability was examined by life/membrane staining reporting comparable viability on planar and nanowire substrates. Imaging the hybrid interface reveals a wrapping of the cell membrane around the very nanowire tip. Patch clamp recordings show similar electrophysiological responses on each type of nanowires compared to planar control substrates. We demonstrate that the morphological characteristic of the nanowire substrate plays a subordinate role which opens up the arena for a large range of nanowire substrates in a functionalized application such as stimulation or sensing.

Details

Authors
  • Jann Harberts
  • Robert Zierold
  • Cornelius Fendler
  • Aune Koitmäe
  • Parisa Bayat
  • Irene Fernandez-Cuesta
  • Gabriele Loers
  • Björn Philipp Diercks
  • Ralf Fliegert
  • Andreas H. Guse
  • Carsten Ronning
  • Gaute Otnes
  • Magnus Borgström
  • Robert H. Blick
Organisations
External organisations
  • University of Hamburg
  • University Medical Center Hamburg-Eppendorf
  • Friedrich Schiller University Jena
  • University of Wisconsin-Madison
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Cell Biology
Original languageEnglish
Pages (from-to)11194-11201
Number of pages8
JournalRSC Advances
Volume9
Issue number20
Publication statusPublished - 2019
Publication categoryResearch
Peer-reviewedYes