Cyclodextrin-surfactant complex: A new route in DNA decompaction

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Abstract

In the present work, we show a new approach for decompaction of DNA-cationic surfactant complexes, e.g., lipoplexes, by using beta-cyclodextrin (beta-CD). The DNA decompaction was achieved by dissolving the surfactant aggregates in the complex by making use of the high affinity between the beta-CD and the free surfactant in solution. The results from fluorescence microscopy and adiabatic compressibility measurements indicate that coils and globules do not coexist. The reported procedure using beta-CD is an efficient way to decompact DNA surfactant complexes because the association constant of surfactants with beta-CD is large. The surfactant's interaction with beta-CD is specific and the nonspecific interaction between beta-CD and biological interfaces is small.

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  • Physical Chemistry
Original languageEnglish
Pages (from-to)772-775
JournalBiomacromolecules
Volume9
Issue number3
Publication statusPublished - 2008
Publication categoryResearch
Peer-reviewedYes