Cyclodextrin-surfactant complex: A new route in DNA decompaction

Research output: Contribution to journalArticle

Standard

Cyclodextrin-surfactant complex: A new route in DNA decompaction. / Gonzalez Perez, Alfredo; Dias, Rita; Nylander, Tommy; Lindman, Björn.

In: Biomacromolecules, Vol. 9, No. 3, 2008, p. 772-775.

Research output: Contribution to journalArticle

Harvard

APA

CBE

MLA

Vancouver

Author

Gonzalez Perez, Alfredo ; Dias, Rita ; Nylander, Tommy ; Lindman, Björn. / Cyclodextrin-surfactant complex: A new route in DNA decompaction. In: Biomacromolecules. 2008 ; Vol. 9, No. 3. pp. 772-775.

RIS

TY - JOUR

T1 - Cyclodextrin-surfactant complex: A new route in DNA decompaction

AU - Gonzalez Perez, Alfredo

AU - Dias, Rita

AU - Nylander, Tommy

AU - Lindman, Björn

PY - 2008

Y1 - 2008

N2 - In the present work, we show a new approach for decompaction of DNA-cationic surfactant complexes, e.g., lipoplexes, by using beta-cyclodextrin (beta-CD). The DNA decompaction was achieved by dissolving the surfactant aggregates in the complex by making use of the high affinity between the beta-CD and the free surfactant in solution. The results from fluorescence microscopy and adiabatic compressibility measurements indicate that coils and globules do not coexist. The reported procedure using beta-CD is an efficient way to decompact DNA surfactant complexes because the association constant of surfactants with beta-CD is large. The surfactant's interaction with beta-CD is specific and the nonspecific interaction between beta-CD and biological interfaces is small.

AB - In the present work, we show a new approach for decompaction of DNA-cationic surfactant complexes, e.g., lipoplexes, by using beta-cyclodextrin (beta-CD). The DNA decompaction was achieved by dissolving the surfactant aggregates in the complex by making use of the high affinity between the beta-CD and the free surfactant in solution. The results from fluorescence microscopy and adiabatic compressibility measurements indicate that coils and globules do not coexist. The reported procedure using beta-CD is an efficient way to decompact DNA surfactant complexes because the association constant of surfactants with beta-CD is large. The surfactant's interaction with beta-CD is specific and the nonspecific interaction between beta-CD and biological interfaces is small.

U2 - 10.1021/bm7012907

DO - 10.1021/bm7012907

M3 - Article

VL - 9

SP - 772

EP - 775

JO - Biomacromolecules

T2 - Biomacromolecules

JF - Biomacromolecules

SN - 1526-4602

IS - 3

ER -