Cytoskeleton components of inside-out and right-side-out plasma membrane vesicles from plants

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Cytoskeleton components of inside-out and right-side-out plasma membrane vesicles from plants. / Sonesson, Anders; Widell, Susanne.

In: Protoplasma, Vol. 177, No. 1-2, 1993, p. 45-52.

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TY - JOUR

T1 - Cytoskeleton components of inside-out and right-side-out plasma membrane vesicles from plants

AU - Sonesson, Anders

AU - Widell, Susanne

N1 - Department affilation moved from v1000887 (CED - Centre for Educational Development) to v1000942 (Division for Higher Education Development) on 2016-03-31 08:48:47.

PY - 1993

Y1 - 1993

N2 - Isolated plasma membrane vesicles purified by aqueous polymer two-phase partitioning were used as a model system for studies on the membrane-associated (cortical) cytoskeleton in plants. Actin, as identified by immunoblotting, was found to be specifically attached to plasma membrane vesicles from cauliflower (Brassica oleracea L.). The actin was not washed off as the vesicles were turned inside-out, indicative of a fairly strong attachment. Triton X-100 extraction of plasma membrane vesicles resulted in an insoluble and hence pelletable fraction where actin could be found together with several other proteins. Our results show that the cortical cytoskeleton is to some extent co-purified with the plasma membrane, and we believe that well defined, inside-out and right-side-out plasma membrane vesicles can be used to study the structure and dynamics of the plant cortical cytoskeleton.

AB - Isolated plasma membrane vesicles purified by aqueous polymer two-phase partitioning were used as a model system for studies on the membrane-associated (cortical) cytoskeleton in plants. Actin, as identified by immunoblotting, was found to be specifically attached to plasma membrane vesicles from cauliflower (Brassica oleracea L.). The actin was not washed off as the vesicles were turned inside-out, indicative of a fairly strong attachment. Triton X-100 extraction of plasma membrane vesicles resulted in an insoluble and hence pelletable fraction where actin could be found together with several other proteins. Our results show that the cortical cytoskeleton is to some extent co-purified with the plasma membrane, and we believe that well defined, inside-out and right-side-out plasma membrane vesicles can be used to study the structure and dynamics of the plant cortical cytoskeleton.

KW - Actin

KW - Cauliflower

KW - Cortical cytoskeleton

KW - Membrane cytoskeleton

KW - Plant plasma membrane.

U2 - 10.1007/BF01403398

DO - 10.1007/BF01403398

M3 - Article

VL - 177

SP - 45

EP - 52

JO - Protoplasma

JF - Protoplasma

SN - 1615-6102

IS - 1-2

ER -