Data processing methods and quality control strategies for label-free LC-MS protein quantification.

Research output: Contribution to journalReview article

Abstract

Protein quantification using different LC-MS techniques is becoming a standard practice. However, with a multitude of experimental setups to choose from, as well as a wide array of software solutions for subsequent data processing, it is non-trivial to select the most appropriate workflow for a given biological question. In this review, we highlight different issues that need to be addressed by software for quantitative LC-MS experiments and describe different approaches that are available. With focus on label-free quantification, examples are discussed both for LC-MS/MS and LC-SRM data processing. We further elaborate on current quality control methodology for performing accurate protein quantification experiments. This article is part of a Special Issue entitled: Computational Proteomics in the Post-Identification Era.

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  • Biological Sciences
Original languageEnglish
Pages (from-to)29-41
JournalBiochimica et biophysica acta
Volume1844
Issue number1
Publication statusPublished - 2014
Publication categoryResearch
Peer-reviewedYes