Decrease in high density lipoprotein cholesterol during prolonged storage. CELL Study Group

Research output: Contribution to journalArticle

Abstract

Different studies on the stability of high density lipoprotein cholesterol (HDL) in frozen serum or plasma have yielded conflicting results, namely increase, decrease, or no change at all during prolonged storage under freezing conditions. As part of a major trial on lipid-lowering strategies we statistically demonstrated a time-related decrease in HDL cholesterol during storage up to 46 months at -20 degrees C. We therefore re-analysed 85 frozen samples that had been analysed fresh and then stored from 26 to 46 months, using the dextran sulphate 500/Mg2+ method. A linear regression analysis of change in HDL cholesterol on time was performed. The slope was significantly negative (p < 0.0005). The regression equation was (decrease in HDL) = 0.05 - 0.008 x (time in months), i.e. after 6 months' storage at -20 degrees C there was almost a 1% decrease in the HDL cholesterol concentration per month of storage.

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Subject classification (UKÄ) – MANDATORY

  • Medicinal Chemistry
  • Public Health, Global Health, Social Medicine and Epidemiology
  • Pharmacology and Toxicology

Keywords

  • analysis, frozen specimen, high density lipoprotein, storage
Original languageEnglish
Pages (from-to)97-101
JournalScandinavian Journal of Clinical & Laboratory Investigation
Volume56
Issue number2
Publication statusPublished - 1996
Publication categoryResearch
Peer-reviewedYes