Degradation of Mannan I and II Crystals by Fungal endo-β-1,4-Mannanases and a β-1,4-Mannosidase Studied with Transmission Electron Microscopy

Research output: Contribution to journalArticle

Abstract

We have used the endo-β-1,4-mannanase from Trichoderma reesei (Tr Man5A), the endo-β-1,4-mannanase from Aspergillus niger (An Man5A) and the exo-β-1,4-mannosidase from A. niger (An Mnd2A) to follow the enzymatic degradation of mannan I and II crystals. The degradation process was studied by transmission electron microscopy and also followed by analysis of the released soluble reducing sugars. The mannan crystals were degraded by the endo-β-1,4-mannanases and to a lesser extent by the exo-β-1,4-mannosidase. The observed hydrolysis pattern on mannan I crystals is fully consistent with the current view of the molecular structure of these crystals. The molecular organization of the mannan chains in mannan II crystals is less clear and the digestion results give some further information about the ultrastructure of mannan II. In addition, insight is provided into the mode of the enzymatic attack on the crystals of mannan I and mannan II.

Details

Authors
  • Per Hägglund
  • Elisabetta Sabini
  • Claire Boisset
  • Keith Wilson
  • Henri Chanzy
  • Henrik Stålbrand
Organisations
Research areas and keywords

Subject classification (UKÄ) – MANDATORY

  • Biological Sciences
Original languageEnglish
Pages (from-to)694-699
JournalBiomacromolecules
Volume2
Issue number3
Publication statusPublished - 2001
Publication categoryResearch
Peer-reviewedYes